OPTIMAL METHODOLOGY FOR DETECTION OF CMV IN CLINICAL LABORATORY

108例咽部和尿液常规病毒培养结果比较 培养和贝壳瓶检测显示贝壳瓶的敏感性为84% 技术。在这项研究中，我们还评估了治疗的有用性 壳中装有地塞米松/二甲基亚砜。我们最初发现这种治疗方法 随着DEX/DMSO的增加，观察到的病毒灶数量增加， 仅用地塞米松治疗是不够的。几种不同的单抗 对抗体染色进行测试，以确定哪种染色最敏感。 来使用。初步比较表明，杜邦单抗IFA优于单抗 Ortho DFA背景染色过多。我们随后比较了 杜邦IFA和西瓦IFA。后者也有非特定的问题 染色，自那以后已从市场上撤下。

A comparison of 108 throat and urine cultures done by conventional viral culture and by shell vial showed 84% sensitivity of the shell vial technique. During this study we also evaluated the usefulness of treating the shell vials with Dexamethasone/DMSO. We initially found that treatment with DEX/DMSO increased the number of viral foci observed and that treatment with DEX alone was insufficient. Several different monoclonal antibody stains were tested to determine which stain was the most sensitive to use. Initial comparison showed the Dupont monoclonal IFA better than the Ortho DFA which had too much background staining. We subsequently compared the Dupont IFA with Syva IFA. The latter also had problems with nonspecific staining and has since been taken off the market.