DEVELOPMENT AND EVALUATION OF A NEW CULTURE MEDIUM FOR YEAST

免疫受损的宿主，包括那些患有艾滋病的患者 或癌症，都容易受到这两种细菌的感染 和真菌。最常见的真菌感染是由酵母引起的。 如念珠菌、曲霉或隐球菌属。尽管这些 微生物在简单的介质上生长良好，如血液、巧克力或 这些媒体不允许轻易地认识到 当可能存在两种或三种不同酵母的混合物时。 这些琼脂上的菌落形态也不容易用来 建议可能存在的酵母种类。为了 简化患者酵母菌的分离和鉴定 样品，我们开发了一种能产生良好酵母生长的培养基。 但这也有助于区分不同种类的酵母 根据菌落形态和颜色，新的培养基将 在与萨布鲁琼脂的比较中被评价为初级 真菌培养的种植基质。此外，媒体将 用作额外的常规血液培养介质，以便 加强血液中酵母菌的检测。

Immunocompromised hosts, including those patients with either AIDS or cancer, are predisposed to infections caused by both bacteria and fungi. The most common fungal infections are caused by yeast such as Candida, Torulopsis or Cryptococcus. Although these organisms grow well on simple media such as blood, chocolate, or Sabouraud's agars, these media do not permit easy recognition of when a mixture of two or three different yeast may be present. Colony morphologies on these agars also cannot be readily used to suggest which species of yeast may be present. In order to facilitate isolation and identification of yeast from patient specimens, we developed a medium which yields good growth of yeast but which also helps differentiate the different species of yeast on the basis of colony morphology and color, The new medium will be evaluated in a comparison with Sabouraud's agar as a primary planting medium for fungal cultures. In addition, the medium will be used as an additional routine blood culture medium in order to enhance the detection of yeast in blood.