INTRACELLULAR MODULATION OF SPECIFIC INTRACELLULAR PROTEINS
INTRACELLULAR MODULATION OF SPECIFIC INTRACELLULAR PROTEINS
批准号:
3752415
负责人:
A RUSSO
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
中文摘要
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英文摘要
We want to regulate expressed proteins within cells at the post-
translational level. Our approach is to provide an alternative to
antisense technology. To accomplish the task we have molecularly re-
engineered the DR1 or DR3 region of gamma globulin heavy chain (HC) gene
fragment by randomization processes to produce a recombinatorial protein
library of approximately 10(12) different immunoglobulin fragments having
different antigen recognition sites. The library can be screened to find
an individual clone that recognizes a given protein by fusing the
recombinant to the M13p8. We have shown that we can isolate a newly
engineered recombinant protein found within the large random library that
binds specifically and tightly to SV40 large T-antigen. In this
particular case the large T-antigen was chosen as a target because the
large T-antigen is a transcriptional factor that has been found to
transform selectively and continually in a mesothelial tumor cell line.
The leader sequence of the HC gene was removed and replaced by either a
nuclear or nucleolar targeting sequence to promote movement of the re-
engineered HC to the nucleus where the T-antigen is functioning. In
addition, oncogenes or transcriptional factors within cancer cells are
potential targets of interest. The method will allow for a new approach
to find yet unknown proteins that contribute to repair of or resistance to
ionizing radiation. This methodology provides a new path to gene therapy
for treatment of cancer or virus infected cells. The project requires
knowledge and use of immunoglobulin gene structure, gene and protein
fusion expression systems, site directed mutagenesis, polymerase chain
reaction, nucleotide sequencing, plasmid and vector construction, protein
screening, and other molecular biologic techniques.
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INTRACELLULAR MODULATION OF SPECIFIC INTRACELLULAR PROTEINS
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批准号:2464488
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
DEVELOPMENT OF SUPEROXIDE DISMUTASE MIMICS
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批准号:3774576
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
RELATIONSHIP OF CELLULAR REDOX STATE AND THERMOTOLERANCE
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批准号:3939515
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
NITROXIDES AS PROTECTORS AGAINST OXIDATIVE STRESS
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批准号:3752336
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
RELATIONSHIP OF CELLULAR REDOX STATE AND THERMOTOLERANCE
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批准号:3963241
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
MECHANISMS OF RADIOPROTECTION
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批准号:4692120
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
PHOTOTHERAPY OF INTRACAVITARY SPACES
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批准号:3916586
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
MOLECULAR BIOLOGY--TISSUE CULTURE CORE FACILITY
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批准号:3854423
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
MOLECULAR BIOLOGY--TISSUE CULTURE CORE FACILITY
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批准号:3896917
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
REDIRECTION OF REDOX PROTEINS TO THE NUCLEUS OF EUKARYOTIC CELLS
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批准号:3752414
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
PHOTOTHERAPY OF INTRACAVITARY SPACES
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批准号:3774572
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
PULSE WAVE EPR SPECTROSCOPY AND IMAGING
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批准号:3774658
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
RESPONSE OF MAMMALIAN CELLS TO CHEMOTHERAPY DRUGS
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批准号:3939509
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
RESPONSE OF MAMMALIAN CELLS TO CHEMOTHERAPY DRUGS
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批准号:3916572
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
PHOTOTHERAPY OF INTRACAVITARY SPACES
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批准号:3853179
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
PULSE WAVE EPR SPECTROSCOPY AND IMAGING
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批准号:3838141
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
PHOTOTHERAPY OF INTRACAVITARY SPACES
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批准号:3838057
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
PHOTOTHERAPY OF MURINE OVARIAN CANCER BY HEMATOPORPHYRIN DERIVATIVE
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批准号:3963251
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
NITROXIDES AS PROTECTORS AGAINST OXIDATIVE STRESS
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批准号:5201260
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
INTRACELLULAR MODULATION OF SPECIFIC INTRACELLULAR PROTEINS
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批准号:6163331
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A RUSSO
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依托单位:
海外基金