BIOCHEMICAL CHANGES IN RATS AS A FUNCTION OF AGE

大鼠的生化变化随年龄的变化

• 批准号: 3757579
• 负责人: J M POSTON
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3757579
• 关键词: acetyl coA acetyltransferase; age difference; aging; alkaline phosphatase; aminoacid; enzyme activity; fatty acids; laboratory rat; lactate dehydrogenases; linolenate

As organisms age there is often decrease in strength and vigor which is reflected in the organism's physical and metabolic activities. Rats, no less than humans, seem to exhibit these characteristics of increasing age. In an effort to examine the biochemistry of some of these changes, male Fisher 344 rats of ages varying from two to twenty-six months were sacrificed and tissues were removed for study. Serum samples from the rats were deproteinized and the free amino acid content was determined. No trend was evident in the concentration of any amino acid even though there was considerable variation from rat to rat. On average, the concentration of any given amino acid was found to be the same in very old rats as it was in very young ones. Several enzyme activities were measured in liver homogenates. Carbonyl reductase (using metyrapone as a substrate) gradually declined with age whereas lactate dehydrogenase remained generally constant. Alkaline phosphatase and thiolase were low in young animals and rose as they reached middle age (until they were about 12-16 months old) and then declined again as the animals became really old. Fatty acid, measured in liver homogenates, were found to increase to middle age and then to decline. Linolenic acid, however, did not vary much with age.

随着生物体年龄的增长，其力量和活力往往会下降， 反映在生物体的身体和代谢活动中。 老鼠，不 比人类更少，似乎表现出这些特征， 年龄 为了研究其中一些变化的生物化学， 雄性Fisher 344只大鼠，年龄从2个月到26个月不等， 处死并取出组织用于研究。 血清标本 将大鼠脱蛋白并测定游离氨基酸含量。 任何氨基酸的浓度都没有明显的趋势， 大鼠之间存在相当大的差异。 平均下来 任何给定氨基酸的浓度被发现是相同的， 就像年轻的老鼠一样。 几种酶活性， 在肝匀浆中测量。 羰基还原酶（使用甲吡酮作为 底物）随着年龄的增长而逐渐下降，而乳酸脱氢酶 基本保持不变。 碱性磷酸酶和硫解酶较低 在年轻的动物和玫瑰，因为他们达到中年（直到他们是 大约12-16个月大），然后随着动物变得 真的很老。 在肝匀浆中测量的脂肪酸被发现 到中年，然后下降。 然而，亚麻酸 不会随着年龄而变化。