BIOCHEMICAL CHANGES IN RATS AS A FUNCTION OF AGE

大鼠的生化变化随年龄的变化

• 批准号: 3779481
• 负责人: J M POSTON
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3779481
• 关键词: acetyl coA acetyltransferase; aging; alkaline phosphatase; animal age group; carbonyl group; creatine kinase; enzyme activity; isocitrate dehydrogenase; laboratory rat; lactate dehydrogenases; liver; metyrapone

As organisms age there is often a decrease in strength and vigor which is reflected in the organism's physical and metabolic activities. Rats, no less than humans, seem to exhibit these characteristics of increasing age. In an effort to examine the biochemistry of some of these changes, male Fisher 344 rats of ages varying from two to twenty-six months were sacrificed and tissues were removed for study. Serum samples from the rats were deproteinized and the free amino acid content was determined. No trend was evident in the concentration of any amino acid even though there was considerable variation from rat to rat. On average, the concentration of any given amino acid was found to be the same in very old rats as it was in very young ones. Several enzyme activities were measured in liver homogenates. Carbonyl reductase (using metyrapone as a substrate) gradually declined with age whereas lactate dehydrogenase remained generally constant. Alkaline phosphatase and thiolase were low in young animals and rose as they reached middle age (until they were about 12-16 months old) and then declined again as the animals became really old.

随着生物体年龄的增长，它们的力量和活力往往会下降