THE OXIDATION OF PROTEINS AND MODEL POLYMERS

L-丙氨酸、L-精氨酸、L-组氨酸、L-赖氨酸和 L-脯氨酸和L-赖氨酸与L-苯丙氨酸的混合聚合物 已经在亚铁和螯合剂的存在下被氧化， 通常是柠檬酸盐。 氧化将羰基引入到 可通过反应转化为仲胺的聚合物 它们与对氨基苯甲酸在钠存在下 氰基硼氢化物。 由此衍生的聚合物表现出特征性的 可以测量的光谱。 聚合物也被切割， 氧化;聚-L-组氨酸似乎特别脆弱 这种攻击。 发现最大的氧化速率 当亚铁与柠檬酸盐的比例为1：2时。

Homopolymers of L-alanine, L-arginine, L-histidine, L-lysine, and L-proline and the mixed polymer of L-lysine and L-phenylalanine have been oxidized in the presence of ferrous iron and a chelator, generally citrate. Oxidation introduces carbonyl groups into the polymer which may be converted into secondary amines by reacting them with p-aminobenzoic acid in the presence of sodium cyanoborohydride. Polymers thus derivatized exhibit characteristic spectra which may be measured. The polymers are also cleaved by the oxidation; poly-L-histidine appears to be especially vulnerable to this sort of attack. The greatest rate of oxidation is found when the ratio of ferrous iron to citrate is 1:2.