METHIONINE SULFOXIDE REDUCTASES

甲硫氨酸亚硫酸还原酶

• 批准号: 5203479
• 负责人: J M POSTON
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5203479
• 关键词: Escherichia coli; animal age group; electron transport; enzyme activity; free radical oxygen; laboratory rat; oxidoreductase; protein purification

Methionine in proteins may be subject to attack by unavoidable reactive oxygen species produced as side products of electron transport process in the cell, forming methionine sulfoxide. An enzyme, methionine sulfoxide reductase, can reverse this oxidation. A method of assay of the reductase has been modified and adapted to measure the activity in rat liver homogenates from animals of various ages. It was found that the specific activity of the reductase gradually dropped so that levels at 24 months were about 80 percent of those found at two months in Fisher 344 rats. This observation parallels that made earlier for carbonyl reductase using metyrapone as substrate. The reductase has been purified from Escherichia coli

蛋氨酸蛋白质可能受到不可避免的反应性攻击， 作为电子传输过程的副产物产生的氧物质 在细胞中形成蛋氨酸亚砜 一种酶，蛋氨酸 亚砜还原酶可以逆转这种氧化。 本发明提供了一种测定以下物质的方法： 还原酶已经被修饰并适于测量 来自不同年龄动物的大鼠肝匀浆。 结果发现 还原酶的比活性逐渐下降， 在24个月的时候，大约有80%的人在两个月的时候被发现。 Fisher 344大鼠。 这一观察结果与早些时候对 使用甲吡酮作为底物的羰基还原酶。 还原酶具有 从大肠杆菌中提纯