QUANTITATIVE ANALYSIS OF VIRAL GENOMES AND THEIR CLINICAL CORRELATIONS

病毒基因组的定量分析及其临床相关性

• 批准号: 3774463
• 负责人: J SHIH
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3774463
• 关键词: HIV infections; communicable disease diagnosis; genetic mapping; genome; hepatitis B; hepatitis B virus group; hepatitis C virus; human immunodeficiency virus; nuclease; nucleic acid sequence; pathologic process; polymerase chain reaction; virus DNA; virus RNA; virus genetics

This is a continuous effort in responding to the increasing demand for more precise measurement of relevant genomic information in any viral infection. The knowledge of the presence of a specific viral gene will help in identifying the infectious agent. However, to assess the stage of a disease, to evaluate the efficacy of a treatment, to determine the value of a predictor in the progression of a disease and to monitor the patient's progression of an infection, a more precise and quantitative analysis of the specific gene would be required. These previously highly research oriented questions can now begin to be answered with the advanced technology of molecular biology such as polymerase chain reaction (PCR), sequencing and mapping of the restriction nuclease digested fragments. We would apply this technology in conjunction with our current on-going clinical research such as hepatitis B virus, hepatitis C virus and HIV infections. We would also improve the basic PCR technique to become a quantitative procedure. We are also looking into PCR technology based genomic typing procedures.

这是一项持续的努力，以应对日益增长的 更精确地测量任何病毒的相关基因组信息 感染。对特定病毒基因存在的了解将会 帮助确定感染源。然而，要评估...的阶段 一种疾病，要评价一种治疗的疗效，要确定其价值 在疾病进展中的预测器，并监测 患者感染的进展，更精确和定量 需要对特定基因进行分析。这些以前的高度 面向研究的问题现在可以开始用高级 聚合酶链式反应(PCR)等分子生物学技术， 限制性核酸酶消化片段的测序和作图。我们 会将这项技术与我们目前正在进行的 乙肝病毒、丙型肝炎病毒和艾滋病毒等临床研究 感染。我们还将改进基本的聚合酶链式反应技术，使其成为 量化程序。我们还在研究基于PCR技术的 基因组分型程序。