QUANTITATIVE ANALYSIS OF VIRAL GENOMES AND THEIR CLINICAL CORRELATIONS

病毒基因组的定量分析及其临床相关性

• 批准号: 3853089
• 负责人: J SHIH
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3853089
• 关键词: HIV infections; genome; hepatitis B; hepatitis B virus group; hepatitis C virus; human immunodeficiency virus; molecular biology; polymerase chain reaction; restriction fragment length polymorphism; restriction mapping; technology /technique; virus genetics

This is a newly initiated program in response to the increasing demand of more precise measurement of relevant genomic information in any viral infection. The knowledge of the presence of specific viral gene will help in identifying the infectious agent. However, to assess the stage of a disease, to evaluate the efficacy of a treatment, to determine the value of a predictor in the progression of a disease and to monitor the patient's progression of an infection, a more precise and quantitative analysis of the specific gene would be required. These previously highly research oriented questions can now being to be answered with the advanced technology of molecular biology such as polymerase chain reaction (PCR), sequencing and mapping of the restriction nuclease digested fragments. We would apply this technology in conjunction with our current on-going clinical research such as hepatitis B virus, hepatitis C virus and HIV infections. We would also improve the basic PCR technique to become a quantitative procedure.

这是一个新发起的计划，以应对日益增长的需求， 更精确地测量任何病毒中的相关基因组信息 感染 了解特定病毒基因的存在将有助于 鉴定传染源的能力然而，要评估一个 疾病，以评估治疗的疗效，以确定的价值， 疾病进展的预测因子，并监测患者的 感染的进展，更精确和定量的分析， 需要特定的基因。 这些先前高度研究 现在可以用先进的技术来回答导向性问题。 分子生物学技术如聚合酶链式反应（PCR）， 限制性核酸酶消化片段的测序和作图。 我们 将这项技术与我们目前正在进行的 临床研究，如B型肝炎病毒、丙型肝炎病毒和艾滋病毒 感染. 我们还将改进基本的PCR技术， 定量程序。