QUANTITATIVE ANALYSIS OF VIRAL GENOMES AND THEIR CLINICAL CORRELATIONS

病毒基因组的定量分析及其临床相关性

• 批准号: 5201144
• 负责人: J SHIH
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5201144
• 关键词: HIV infections; communicable disease diagnosis; genetic mapping; genome; hepatitis B; hepatitis B virus group; hepatitis C virus; human immunodeficiency virus; human tissue; nuclease; nucleic acid sequence; pathologic process; polymerase chain reaction; virus DNA; virus RNA; virus genetics

This is a continuous effort in response to the increasing demand of more precise measurement of relevant genomic information in any viral infection. The knowledge of the presence of specific viral gene will help in identifying the infectious agent. However, to assess the stage of a disease, to evaluate the efficacy of a treatment, to determine the value of a predictor in the progression of a disease and to monitor the patient's progression of an infection, a more precise and quantitative analysis of the specific gene would be required. These previously highly research oriented questions can now begin to be answered in the routine clinical laboratories with the advanced technology of molecular biology such as polymerase chain reaction (PCR), and the sequencing and mapping of the restriction nuclease digested fragments. We have adopted many of the published proven procedures and others, we initiated our own research to meet our needs. Then, we applied these technologies in conjunction with our current ongoing clinical research such as hepatitis B virus, hepatitis C virus and HIV infections. We have also improved the basic PCR technique to become a semi-quantitative procedure. We also conducted a genomic typing study based on PCR technology for HCV infected blood donors and post transfusion hepatitis C patients. During FY95, we were able to apply the same principles of using PCR a primary study tool for viral infection to a newly identified human hepatitis virus, HGV. This new hepatitis virus was identified through a joint effort of our group at NIH, scientists at CDC, and Genelab of California under a CRADA agreement. This virus was found to be causing post-transfusion hepatitis. Specific HGV RNA was identified in both recipients and paired donors' sera. It can cause chronic infection with mild or even no observed liver function abnormality. The prevalence in blood donors, in the preliminary studies, was higher than that of HCV.

这是一项持续的努力，以应对日益增长的需求， 精确测量任何病毒中的相关基因组信息 感染了解特定病毒基因的存在将有助于 鉴定传染源的能力然而，要评估一个 疾病，以评估治疗的疗效，以确定价值 疾病进展的预测因子，并监测 患者的感染进展，一个更精确和定量的 需要对特定基因进行分析。这些以前高度 面向研究的问题现在可以开始在例行程序中得到回答 拥有先进分子生物学技术的临床实验室 例如聚合酶链反应（PCR），以及 限制性核酸酶消化的片段。我们采用了许多 发表了经过验证的程序和其他程序，我们开始了自己的研究， 满足我们的需求。然后，我们将这些技术与 我们目前正在进行的临床研究，如B型肝炎病毒， C病毒和HIV感染。我们还改进了基本的PCR技术 成为一个半定量的过程。我们还进行了基因组 HCV感染献血员的PCR分型研究 输血后丙型肝炎患者。 在1995财政年度，我们能够应用与使用PCR相同的原则， 病毒感染新发现人类的主要研究工具 肝炎病毒HGV这种新的肝炎病毒是通过一种 我们在NIH的团队，CDC的科学家和Genelab的共同努力， 加州根据CRADA协议。这种病毒会导致 输血后肝炎HGV RNA特异性在两种组织中均被发现。 受体和配对供体的血清。它可以引起慢性感染， 肝功能轻度异常或甚至无肝功能异常。的患病率 献血员的HCV感染率高于HCV感染者。