QUANTITATIVE ANALYSIS OF VIRAL GENOMES AND THEIR CLINICAL CORRELATIONS

病毒基因组的定量分析及其临床相关性

• 批准号: 3837959
• 负责人: J SHIH
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3837959
• 关键词: HIV infections; genome; hepatitis B; hepatitis B virus group; hepatitis C virus; human immunodeficiency virus; molecular biology; polymerase chain reaction; restriction fragment length polymorphism; restriction mapping; technology /technique; virus genetics; virus infection mechanism

This is a continuation of a recently initiated program in responding to the increasing demand of more precise measurement of relevant genomic information in any viral infection. The knowledge of the presence of specific viral gene will help in identifying the infectious agent. However, to assess the stage of a disease, to evaluate the efficacy of a treatment, to determine the value of a predictor in the progression of an infection, a more precise and quantitative analysis of the specific gene would be required. These previously highly research oriented questions can now begin to be answered with the advanced technology of molecular biology such as polymerase chain reaction (PCR), sequencing and mapping of the restriction nuclease digested fragment. We would apply the technology in conjunction with our current on-going clinical research such as hepatitis B virus, hepatitis C virus and HIV infections. We will also improve the basic PCR technique to become a quantitative procedure.

这是最近发起的一个项目的延续， 对相关基因组的更精确测量的需求不断增加 任何病毒感染的信息。 知道存在的 特定的病毒基因将有助于识别传染源。 然而，为了评估疾病的阶段，为了评估药物的疗效， 治疗，以确定在进展中的预测值， 感染，对特定基因进行更精确和定量的分析， 将被要求。 这些以前高度研究导向的问题可以 现在开始用先进的分子生物学技术来回答 如聚合酶链反应（PCR）、测序和绘制基因组图谱。 限制性核酸酶消化的片段。 我们将把这项技术应用于 结合我们目前正在进行的临床研究，如肝炎 B病毒、丙型肝炎病毒和艾滋病毒感染。 我们还将完善 基本的PCR技术，成为一个定量的程序。