RADIOLYSIS, PHOTOLYSIS AND SONOLYSIS OF CELLS AND THEIR CONSTITUENTS

细胞及其成分的放射分解、光分解和声分解

• 批准号: 3774571
• 负责人: P RIESZ
• 金额: --
• 依托单位: DIVISION OF CANCER TREATMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3774571
• 关键词: alcohols; antineoplastics; antioxidants; combination cancer therapy; cyclohexanes; cytolysis; dimethylsulfoxide; dioxins; electron spin resonance spectroscopy; formamide; free radical scavengers; neoplasm /cancer photoradiation therapy; neoplasm /cancer radiation therapy; neoplasm /cancer therapy; nitroalkane; photolysis; photosensitizing agents; radiosensitizer; superoxide dismutase; toluene; ubiquinone; ultrasound biological effect; ultrasound therapy; water solution

The chemical effects of ultrasound in aqueous solutions and organic liquids are being studied as model systems. Ultrasound is used to produce hyperthermia in radiation therapy and a new approach, sonodynamic cancer therapy, seeks to take advantage of the synergistic effects of ultrasound and anticancer drugs (eg., adriamycin). Electron spin resonance and spin trapping methods were used to investigate the sonochemistry and antioxidant properties of ubiquinone (Coenzyme Qo) in aqueous solutions. Evidence for the formation of superoxide radicals in oxygen-free argon-saturated aqueous solutions was obtained since Superoxide Dismutase (SOD) inhibits the sonochemical formation of ubiquinol. Spin trapping studies with 3,5-dibromo-4-nitrosobenzene sulfonate (DBNBS) established the formation of 5-yl radicals by addition of OH radicals to the 5,6 double bond of ubiquinone. Pyrolysis products such as methyl radicals were also formed. Conclusive evidence for the formation of superoxide radicals formed by 50 kHz sonolysis of oxygen free argon-saturated water was obtained by means of the SOD-inhibited reduction of cytochrome c. Superoxide is formed by radical reactions as a result of the very high temperatures (approx. 5000 K) in collapsing cavitation bubbles. 2,4,6-tri-t. butylnitrosobenzene (3tBNB) was found to be a reliable spin trap for investigating the sonolysis of organic liquids. Thus, similar results were found in the sonolysis of dimethylformamide using DBNBS and 3tBNB as the spin traps. The sonolysis of a variety of organic liquids, including n-alkanes, n-alcohols, dioxane, cyclohexane and toluene were investigated. In the homologous series of n-alkanes and n-alcohols the spin adduct yields with 3tBNB increase with decreasing vapor pressure as predicted from the thermal hot-spot theory of sonochemistry.

超声波在水溶液和有机介质中的化学效应 液体正被作为模型系统进行研究。超声波被用来 在放射治疗中产生高温和一种新的方法--声动力学 癌症治疗，寻求利用 超声波和抗癌药物(如阿霉素)。电子自旋 用共振和自旋俘获方法研究了 辅酶QO的声化学及抗氧化性 水溶液。超氧阴离子自由基形成的证据 获得了无氧的Ar饱和水溶液， 超氧化物歧化酶(SOD)抑制声化学形成 泛喹酚。3，5-二溴-4-亚硝基苯的自旋捕获研究 磺酸盐(DBNBS)通过加成建立了5-基自由基的形成 羟基自由基对泛醌的5，6双键的作用。热解产物 如甲基自由基也形成了。确凿的证据表明 50 kHz声解氧形成超氧自由基的研究 用超氧化物歧化酶抑制的方法获得了含Ar的游离水 细胞色素c.超氧化物的还原是由自由基反应形成的 由于非常高的温度(约5000 K)正在坍塌 空化气泡。2，4，6-三-t.发现了丁基亚硝苯(3tBNB) 是研究有机分子超声分解的可靠自旋陷阱 液体。因此，在超声波分解中也发现了类似的结果。 二甲基甲酰胺使用DBNBS和3tBNB作为自旋陷阱。超声波消解 各种有机液体，包括正构烷烃，正构醇， 对二氧六环、环己烷和甲苯进行了研究。在同源的 正构烷烃和正构醇系列与3tBNB的自旋加合物 随着蒸汽压的下降而增加，从热力预测 声化学热点理论。