RADIOLYSIS, PHOTOLYSIS AND SONOLYSIS OF CELLS AND THEIR CONSTITUENTS

细胞及其成分的放射分解、光分解和声分解

• 批准号: 3916583
• 负责人: P RIESZ
• 金额: --
• 依托单位: DIVISION OF CANCER TREATMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3916583
• 关键词: DNA; antineoplastics; combination cancer therapy; cytolysis; daunorubicin; doxorubicin; electron spin resonance spectroscopy; free radicals; hydroxyl group; ionizing radiation; light adverse effect; neoplasm /cancer photoradiation therapy; neoplasm /cancer thermotherapy; nonvisual photosensitivity; photochemistry; quinoline; radiation sensitivity; singlet oxygen; superoxides; temperature; tissue /cell culture; ultrasound; ultrasound biological effect; ultraviolet radiation

The photochemistry of the antitumor drugs adriamycin, daunomycin, certain quinolinium salts, and gilvocarcin V was investigated in relation to the possible combination of chemotherapy with photodynamic therapy. By the use of electron spin resonance and spin trapping techniques, and of (17-0) enriched water and oxygen, the relative contributions of superoxide radicals, hydroxyl radicals, and singlet oxygen to the photochemistry of these antitumor drugs was elucidated. The chemical effects of ultrasound were studied in relation to hyperthermia (used in combination with radiation therapy) and the possible consequences of diagnostic and therapeutic applications. The effect of varying the temperature of cavitation bubbles in the presence of different rare gases on free radical formation and the shearing stress induced by ultrasound on mammalian cells was measured. The hydroxyl radical yields were in the order xenon>kryton>argon>neon>helium in accord with the higher temperatures of the cavitation bubbles. However, mammalian cell lysis induced by shearing stress was the same for all rare gases and independent of their thermal conductivity and the temperature of the cavitation bubbles. The radicals generated in aqueous solutions of nucleic acid and protein components by ultrasound have been identified by spin trapping and compared with those formed by ionizing radiation and by UV-photolysis in the presence of hydrogen peroxide. The observed isotopic exchange reactions suggest the possible use of sonochemical methods for the synthesis of deuterated protein and nucleic acid constituents.

抗肿瘤药物阿霉素、道诺霉素、 某些喹啉鎓盐和 gilvocacin V 已在 与化疗与可能的联合治疗的关系 光动力疗法。 通过使用电子自旋共振和 自旋捕获技术，以及（17-0）富水和氧气， 超氧自由基、羟基的相对贡献 自由基和单线态氧对这些光化学的影响 抗肿瘤药物得到阐明。 超声波的化学效应 进行了与热疗相关的研究（与 放射治疗）以及诊断和治疗可能产生的后果 治疗应用。 改变温度的影响 不同稀有气体存在下的空化气泡 自由基的形成和剪切应力 测量了哺乳动物细胞上的超声波。 羟基自由基 产率顺序为氙>氪>氩>氖>氦 随着空化气泡的温度升高。 然而， 剪切应力诱导的哺乳动物细胞裂解与 所有稀有气体，与其热导率无关， 空化气泡的温度。 生成的自由基 在核酸和蛋白质组分的水溶液中 超声波已被自旋捕获识别并与 那些由电离辐射和紫外线光解形成的 过氧化氢的存在。 观测到的同位素交换 反应表明可以使用声化学方法 氘化蛋白质和核酸成分的合成。