STRUCTURE-FUNCTION STUDIES OF THE PLATELET-DERIVED GROWTH FACTOR AND RECEPTOR

血小板衍生生长因子（PDGF）是一种有效的促细胞分裂剂， 间充质起源 为了阐明结构-功能 配体-受体和受体信号分子之间的关系， PDGF受体的细胞外结构域、细胞内结构域、细胞外结构域和细胞外结构域。 酪氨酸激酶和PI-3激酶的Src同源区2（SH 2），以及 GAP已经被表达出来。 蛋白质已被纯化用于结构分析。 功能分析。 目前，已经表明N-SH 2 PI-3激酶的结构域通过钙依赖性结合α PDGFR， 机制（手稿提交给J Biol Chem，1993）。 此外，一个大 对于X射线晶体学和 核磁共振波谱学已经被应用。 目前， 角质形成细胞生长因子（KGF）的三维结构， KGF的突变体正在分析中。 的分子具有 在计算机图形系统上建立模型，基于三个- 成纤维细胞生长因子（FGF）的三维结构。

Platelet-derived growth factor (PDGF) is a potent mitogen to cells of mesenchymal origin. In order to elucidate the structure-function relationships between ligand-receptor and receptor-signaling molecules, the extracellular domains of the PDGF receptor, the intracellular tyrosine kinase and the Src-homology region 2 (SH2) of PI-3 kinase and GAP have been expressed. The proteins have been purified for structural and functional analysis. Currently, it has been shown that the N-SH2 domain of PI-3 kinase binds the alphaPDGFR through a calcium dependent mechanism (manuscript submitted to J Biol chem, 1993). further, a large proportion of the proteins expressed for both x-ray crystallography and nuclear magnetic resonance spectroscopy have been committed. Currently, the three-dimensional structure of keratinocyte growth factor (KGF) and a mutant of KGF are in the process of being analyzed. the molecules have been model built on a computer graphics system based on the three- dimensional structure of fibroblast growth factor (FGF).