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NEW CLONING SYSTEM FOR TUMOR SUPPRESSOR/GROWTH INHIBITORY GENES

NEW CLONING SYSTEM FOR TUMOR SUPPRESSOR/GROWTH INHIBITORY GENES
肿瘤抑制/生长抑制基因的新克隆系统
批准号：
3774922
负责人：
S A AARONSON
金额：
$0.0万
依托单位：
DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3774922
关键词：
DNA replication Epstein Barr virus clone cells complementary DNA gene expression human tissue method development molecular cloning neoplasm /cancer genetics neoplastic cell phenotype plasmids transfection /expression vector tumor suppressor genes

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英文摘要
A new expression cloning system for isolating tumor suppressor genes and growth inhibitory genes has been developed. The main components of this system are a regulated expression of cloned cDNA and episomal maintenance of transfected plasmid. The regulated expression system offers an essential tool for a transition between normal and tumorigenic phenotypes. The episomal replication system was achieved by using the replication system of Epstein-Barr virus. The transfection efficiency of human tumor cells was greatly increased using this system. Plasmid rescue from transfected human tumor cells will provide an advantage for analyzing positive clones.
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