ONCOGENIC ACTIVATION OF EGF RECEPTOR AND RELATED GENES

EGF 受体和相关基因的致癌激活

• 批准号: 3963545
• 负责人: S A AARONSON
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3963545
• 关键词: Abelson leukemia virus; avian leukosis virus; cell growth regulation; epidermal growth factor; genetic library; genetic manipulation; genetic mapping; interleukin 2; interleukin 3; molecular biology; molecular cloning; mouse sarcoma virus; oncogenes; tissue /cell culture; virus genetics

The mechanisms by which genes normally encoding for growth factor receptors can be "activated" as oncogenes is being studied. A molecular clone of the v-erb gene from the ES4 isolate of avian erythroblastosis virus (AEV) has been constructed into a murine retroviral vector (MuLV) and a virus has been obtained after transfection of this DNA into NIH/3T3 cells and "rescued" with a helper leukemogenic virus. This new murine pseudotype of the v-erb gene is now being used to study how it alters the growth properties and the differentiation program of a number of in vitro systems of murine orgin. Molecular constructions have been engineered into retroviral vectors to test the transforming potential of the recently isolated HER 2 gene (also called neu). The amino truncation of the protein "activates" its transforming ability; a double amino and carboxy truncation does not enhance this latter effect (as suggested in other systems). Virus stocks obtained as described above from this transforming counterpart of the HER 2 gene are now being tested in vivo and in vitro.

基因通常编码生长因子受体的机制