DEVELOPMENT OF NANOLITER CAPILLARY LC/MS TECHNIQUES

纳升毛细管 LC/MS 技术的开发

• 批准号: 3777530
• 负责人: K B TOMER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3777530
• 关键词: analytical method; biomedical equipment development; electrospray ionization mass spectrometry; environmental contamination; gas chromatography; liquid chromatography; mass spectrometry; penicillins; peptides; pulmonary surfactants; zonal electrophoresis

A perennial problem in the mass spectrometric analysis of both biological and environmental samples is that the absolute level of analyte is extremely low. One approach to this problem is to develop low volume- high flux delivery systems for the mass spectrometer. We have undertaken the development of interfaces for nanoscale packed capillary LC and MS. These capillary systems offer the same advantages over wider-bore LC systems that capillary GC offers over packed-column GC, a high flux of analyte into the MS but with a significantly lower total analyte level necessary. Current developments are in the areas of nanoscale capillary LC and capillary zone electrophoresis interfaced with both coaxial continuous flow fast atom bombardment MS and electrospray ionization MS, including electrospray ionization on a magnetic sector instrument. We have applied these techniques to separations of lipoproteins such as palmitoylated lung surfactant protein SP-C and other fatty acid acylated peptides, to Edman degradation peptides, to complex mixtures of proteins and to mixtures of penicillins.

这是生物质谱分析中一个长期存在的问题