COLLABORATIVE PROJECTS IN ENVIRONMENTAL HEALTH SCIENCES

环境健康科学合作项目

• 批准号: 3876935
• 负责人: K B TOMER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3876935
• 关键词: bioassay; bradykinin; chemical carcinogen; chemical carcinogenesis; dairy products; eicosanoid metabolism; eicosanoids; electrophoresis; environmental health; gas chromatography mass spectrometry; growth factor; high performance liquid chromatography; laboratory mouse; liver cells; mass spectrometry; prostaglandins; toxin metabolism

Collaborative projects in the environmental health sciences includes those projects in which the mass spectrometry work-group collaborates with other groups, both within and without the institute to solve problems of mutual interest. A major focus of these projects is the determination of xenobiotics in the environment, in living systems and the determination of their metabolic products. Their importance lies in the fact that the adverse interactions between compounds in the environment and their metabolite with living systems underlie our health concerns. These projects typically involve on-line separation and identification of complex mixtures and often involve use of all instrumental techniques available in the MS lab including thermospray LC/MS (TSP/LC/MS), FAB/MS and FAB/MS/MS (including the use of continuous flow techniques) and GC/MS. A typical project is the identification of the metabolites of H.C. Blue No. 1 and H.C. Blue No. 2. H.C. Blue No. 1 is a known carcinogen which differs only slightly from the non-carcinogenic H.C. Blue No. 2. The metabolic profiles in mice of these two compounds differ significantly. H.C. Blue 2 was observed to yield one major metabolic product. Using GC/MS, HR/MS and TSP/MS technique, this product has been identified as a side chain oxidation product. In contrast, the carcinogenic H.C. Blue 1 undergoes dealkylation to the free amine, from which a variety of metabolites arise. The free aromatic amines are known carcinogens. Therefore, the difference in carcinogenic behavior can be explained on the basis of their different metabolisms. Other projects, which are included in this heading, include analysis of airway epithelium prostaglandins, the determination of bradykinin in bovine milk, the identification of liver cell growth factor, and the identification of the products of free radicals with spin traps.

环境健康科学方面的合作项目包括 质谱工作组与其他机构合作的项目 团体，无论是内部和外部的研究所，以解决相互的问题， 兴趣这些项目的主要重点是确定 生物系统中的外源性物质以及 它们的代谢产物它们的重要性在于， 环境中的化合物与其 代谢物与生命系统的关系是我们健康问题的基础。这些项目 典型地涉及复杂混合物的在线分离和识别 并且经常涉及使用MS中可用的所有仪器技术 实验室，包括热喷雾LC/MS（TSP/LC/MS）、FAB/MS和FAB/MS/MS （包括使用连续流动技术）和GC/MS。 一个典型的项目是鉴定H.C.的代谢产物。Blue No. 1和H.C.蓝色2号。H.C.蓝色1号是一种已知的致癌物质， 只有轻微的非致癌性HC蓝色2号。代谢 这两种化合物在小鼠中的分布显著不同。H.C.蓝2 观察到产生一种主要代谢产物。使用GC/MS、HR/MS和 采用TSP/MS技术，本品已被鉴定为侧链 氧化产物相反，致癌的H.C.蓝色1号 脱烷基化为游离胺，由此产生多种代谢物。 游离芳香胺是已知的致癌物质。因此，差异 在致癌行为的基础上可以解释他们的不同 新陈代谢 列入本标题的其他项目包括分析 牛气道上皮细胞缓激肽，测定 牛奶中肝细胞生长因子的鉴定， 用自旋陷阱鉴定自由基的产物。