APPLICATION OF THERMOSPRAY LC-MS TO STRUCTURE ELUCIDATION OF BIOMOLECULES

热喷雾液质联用技术在生物分子结构解析中的应用

• 批准号: 3918702
• 负责人: K B TOMER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3918702
• 关键词: acetylcysteine; chemical carcinogen; chemical structure function; cysteine; glutathione; mass spectrometry; microsomes; molecular biology

The majority of biochemical samples are complex mixtures. Separation and isolation of each component prior to analysis is often laborious and tedious. Therefore, considerable effort has been expended on the development and application of methodologies designed to separate and introduce each separated component of a mixture directly into a mass spectrometer. GC/MS, used for the separation and analysis of volatile and thermally stable substances is a relatively mature technique. LC/MS, which combines liquid chromatographic separation with MS analysis is still at a stage where several interfaces are in popular use and where the capabilities of the technique in its various manifestations have not been thoroughly explored. We are currently exploring the applicability of thermospray (TSP) as an LC-MS interface for biochemical problems. Several studies are being carried out concurrently including: 1) determination of glutathione and related ccnjugates (cysteine, N-acetylcystene) of exogenous chemicals; 2) analysis of the highly toxic senecionine alkaloids and their microsomal metabolites: 3) analysis of the herbicide metribuzine and its plant metabolites; 4) ANF (alpha- naphthoflavone) metabolites; and 5) the metabolic products of H.C. Blue No. 1 and H.C. H.C. Blue No. 2 (H.C. Blue No. 1 is a carcinogen previously used in hair dye.

大多数生化样品是复杂的混合物。 在分析之前， 通常是费力和乏味的。 因此，作出了相当大的努力， 用于制定和应用方法 设计用于分离和引入 混合物直接进入质谱仪。 GC/MS，用于 挥发性和热稳定性物质的分离和分析 是一种相对成熟的技术。 LC/MS，结合液体 色谱分离与质谱分析仍处于一个阶段， 其中几个接口被广泛使用， 该技术在其各种表现形式中的能力 没有被彻底探索。 我们目前正在探索热喷雾（TSP）的适用性 作为生化问题的LC-MS接口。 几项研究 同时进行的工作包括：1）确定 谷胱甘肽和相关共轭物（半胱氨酸，N-乙酰半胱氨酸） 外源性化学物质; 2）高毒性物质的分析 生物碱及其微粒体代谢物：3）分析 除草剂嗪草酮及其植物代谢物; 4）ANF（α- （5）H.C. 蓝色1号和H.C. H.C.蓝色2号（H.C.蓝色1号是一个 致癌物质，以前用于染发剂。