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CENTER FOR FAST KINETICS RESEARCH

快速动力学研究中心

基本信息

批准号：
3103699
负责人：
ANTHONY M HARRIMAN
金额：
$ 0.12万
依托单位：
UNIVERSITY OF TEXAS AT AUSTIN
依托单位国家：
美国
项目类别：
财政年份：
1975
资助国家：
美国
起止时间：
1975-06-01 至 1994-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3103699
关键词：
biomedical equipment resource confocal scanning microscopy flash photolysis fluorescence spectrometry nuclear medicine online computer pulse radiolysis

项目摘要

During the next funding period the Center for Fast Kinetics Research (CFKR) will continue to develop its sophisticated array of computerized instruments for the generation and measurement of transient species. These experimental capabilities, together with the expertise of the CFKR staff, will be made available to the scientific community. The CFKR will advertise its capabilities in an effort to open the methods of fast kinetics to as wide an audience as possible. To this end, the CFKR has appointed a new advisory board, demonstrating far greater responsibility and involvement than apparent in earlier years. In order to address specific scientific problems, e.g., rapid energy and electron transfers in biological and biomimetic systems, time-resolved fluorescence from living cells and other organisms, characterization of transient species in opaque materials, observation of weakly-absorbing intermediates, selective excitation of chromophores in proteins, measurement of the energy content of transient species, etc., the CFKR seeks to upgrade and extend its instrumentation. In particular, new detection methods will be implemented consisting of diffuse reflectance spectroscopy, photoconductivity, thermal lensing, and photo-acoustic calorimetry. Substantial modifications will be made in the area of time-resolved fluorescence spectroscopy, in order to improve its existing reliability and resolution (both temporal and spectral). Construction of a novel confocal microscope will be undertaken; this instrument will permit video scanning of the specimen via a linear servo galvanometer system, photoablation through an acousto-optic modular, and time-resolved fluorescence spectroscopy throughout the entire UV-VIS spectrum with time and wavelength resolutions of 5 ps and 5 nm respectively. The available facilities for preparation and handling of biological samples will be extended. Also, control of all experiments will be transferred from the existing PDP 11/70 minicomputer to a dedicated array of microcomputers, thus minimizing down-time, increasing software flexibility, and removing the need for costly maintenance agreements. Further aims for the next funding period include the undertaking of interdisciplinary collaborations with outside researchers in fields relating to biomedical problems, maintaining a close watch for searchers in fields relating to biomedical problems, maintaining a close watch for technological advances of relevance in the field of fast kinetics, and ensuring that the biomedical fraternity are kept fully informed our resources and capabilities.

在下一个资助期间，快速动力学研究中心（CFKR）将继续开发其先进的计算机化用于产生和测量瞬态物质的仪器。这些实验能力，加上CFKR的专业知识，工作人员，将提供给科学界。CFKR将宣传其能力，努力打开快速的方法，尽可能广泛地传播给观众。为此，CFKR已任命了一个新的顾问委员会，和参与比前几年明显。为了解决具体的科学问题，例如，快速能量和电子转移在生物和仿生系统中，活细胞和其他生物体，瞬时物种的表征在不透明材料中，观察到弱吸收中间体，蛋白质中发色团的选择性激发，瞬态物质的能量含量等，CFKR寻求升级，扩展其仪器。特别是，新的检测方法将实施包括漫反射光谱，光电导、热透镜和光声量热法。将在时间分辨领域作出重大修改荧光光谱，以提高其现有的可靠性和分辨率（时间和光谱两者）。小说的结构将采用共聚焦显微镜;该仪器将允许视频通过线性伺服检流计系统扫描样品，通过声光模块进行光消融，整个UV-VIS光谱中随时间的荧光光谱波长分辨率分别为5ps和5nm。可用将配备制备和处理生物样本的设施延长此外，所有实验的控制权将从现有的PDP 11/70小型计算机到专用的微型计算机阵列，从而最大限度地减少停机时间，增加软件灵活性，需要昂贵的维护协议。下一个目标资助期间包括跨学科的承诺与生物医学相关领域的外部研究人员合作问题，保持密切关注搜索者在有关领域，生物医学问题，密切关注技术进步在快速动力学领域的相关性，并确保生物医学兄弟会保持充分了解我们的资源，能力的