INHIBITION OF GENE EXPRESSION BY NORMAL & MODIFIED ANTISENSE OLIGONUCLEOTIDES
INHIBITION OF GENE EXPRESSION BY NORMAL & MODIFIED ANTISENSE OLIGONUCLEOTIDES
批准号:
3792518
负责人:
C J MARCUS-SEKURA
金额:
$0.0万
依托单位:
--
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
关键词:
Epstein Barr virus antisense nucleic acid antiviral agents chloramphenicol acetyltransferase enzyme activity enzyme linked immunosorbent assay gene expression genetic regulation high performance liquid chromatography immunofluorescence technique monoclonal antibody nucleic acid chemical synthesis nucleic acid sequence oligonucleotides phorbols protein purification tissue /cell culture transfection virus antigen
中文摘要
这个项目最初是为了确定是否
英文摘要
This project was initially undertaken in order to determine whether
modified oligonucleotides of appropriate antisense sequence can inhibit
biological reactions, and to examine their potential as antiviral
agents. A transient expression system in eukaryotic cells was used to
see whether normal and modified oligonucleotides could inhibit the
activity of the enzyme chloramphenicol transferase CAT (the gene for CAT
was introduced into the cells by DNA transfection). It was determined
that micromolar concentrations of modified oligonucleotides could
inhibit CAT activity and that chain length, concentration, and nature of
the chemical modification of the oligonucleotide could influence the
degree of inhibition. This work has been published (1987) as well as a
second paper discussing techniques for using antisense oligonucleotides
has been published (1988). Experiments using antisense oligonucleotides
have now been extended to study potential antiviral effects using EBV
gene expression as a model. Antisense molecules appear to inhibit
expression of a specific EBV early antigen, EA(D), induced by
stimulation of B95-8 cells with the phorbol ester TPA as assayed by
immunofluorescence and ELISA using a monoclonal antibody to EA(D).
Additional antisense sequences are being examined to determine the
specificity of this inhibition. We have purchased an instrument to
synthesize oligonucleotides for these experiments and J. Smith has
recently joined the laboratory, which should help greatly to supply
materials which have been a limiting factor in this project. We have
developed protocols for large-scale synthesis of antisense
oligonucleotides and purification by HPLC, and implementation is planned
in the near future. Temporarily, active research in this area has been
curtailed due to time and personnel constraints.
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IMMUNOLOGIC ANALYSIS OF A HERPES VIRUS GLYCOPROTEIN VACCINE CANDIDATE--EBV GP350
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批准号:3770329
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:C J MARCUS-SEKURA
-
依托单位:--
DETECTION OF VIRUSES IN CELL SUBSTRATES
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批准号:3792523
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C J MARCUS-SEKURA
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依托单位:--
EXPRESSION OF HIV ANTIGENS IN E COLI
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批准号:3811240
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C J MARCUS-SEKURA
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依托单位:--
DETECTION OF VIRUSES IN CELL SUBSTRATES
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批准号:3804796
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C J MARCUS-SEKURA
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依托单位:--
EXPRESSION OF HIV ANTIGENS IN E COLI
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批准号:3804789
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C J MARCUS-SEKURA
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依托单位:--
ANALYSIS OF THE HIV INTEGRATION REACTION USING RECOMBINANT INTEGRASE
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批准号:3770330
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C J MARCUS-SEKURA
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依托单位:--
EXPRESSION OF HIV ANTIGENS IN E COLI
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批准号:3792517
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C J MARCUS-SEKURA
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依托单位:--
INHIBITION OF GENE EXPRESSION BY NORMAL & MODIFIED ANTISENSE OLIGONUCLEOTIDES
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批准号:3804790
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C J MARCUS-SEKURA
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依托单位:--
EPITOPE MAPPING OF THE EBV MEMBRANE ANTIGEN
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批准号:3804788
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C J MARCUS-SEKURA
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依托单位:--
DETECTION OF VIRUSES IN CELL LINES USED TO MANUFACTURE BIOLOGICAL PRODUCTS
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批准号:3770331
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C J MARCUS-SEKURA
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依托单位:--
EPITOPE MAPPING OF THE EBV MEMBRANE ANTIGEN
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批准号:3811239
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C J MARCUS-SEKURA
-
依托单位:--
INHIBITION OF GENE EXPRESSION BY NORMAL & MODIFIED ANTISENSE OLIGONUCLEOTIDES
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批准号:3811241
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项目类别:
-
资助金额:$0.0万
-
财政年份:--
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负责人:C J MARCUS-SEKURA
-
依托单位:--
ANALYSIS OF THE HIV INTEGRATION REACTION USING RECOMBINANT INTEGRASE
-
批准号:3748159
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项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:C J MARCUS-SEKURA
-
依托单位:--
EPITOPE MAPPING OF THE EBV MEMBRANE ANTIGEN
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批准号:3792516
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项目类别:
-
资助金额:$0.0万
-
财政年份:--
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负责人:C J MARCUS-SEKURA
-
依托单位:--
DETECTION OF VIRUSES IN CELL SUBSTRATES
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批准号:3811247
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C J MARCUS-SEKURA
-
依托单位:--
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