CHRONOLOGY OF APPEARANCE OF HCV-RNA AND ANTIBODIES IN HCV INFECTION

HCV-RNA的出现顺序和不同抗体的出现顺序 两种实验性感染后的丙型肝炎病毒（HCV）表位 感染HCV的黑猩猩，已经被研究过了。 这些黑猩猩被注入了 使用来自非反应性捐献者的未处理合并血浆 抗-HCV。 输注前采集血清样本，然后 输注后每周一次。 对这些样本进行抗HCV检测， HBsAg、抗-HBs、抗-HBc和丙氨酸转氨酶（ALT）。 血清 用聚合酶链检测HCV-RNA 反应测定（PCR）和针对c100-3、C22-3、c33 c和5-1-1的抗体 免疫印迹RIBA II试验 两只黑猩猩表现出不同的 HCV-RNA和HCV-RNA的序列和时间出现模式 针对不同HCV表位的抗体。 HCV特异性基因组 在一个受试者中，在输注后一周内在血清中检测到序列。 动物和9周的另一只黑猩猩。 ALT值升高是 在两只动物输注后3周观察。

The sequence of appearance of HCV-RNA and of the different antibodies to hepatitis C virus (HCV) epitopes following experimental infection of two chimpanzees with HCV, have been investigated. The chimps were infused with unprocessed, pooled plasma from donations that were non-reactive for anti-HCV. Serum samples were obtained prior to infusion, and then weekly subsequent to infusion. These samples were tested for anti-HCV, HBsAg, anti-HBs, anti-HBc and alanine aminotransferase (ALT). Serum samples were also tested for the presence of HCV-RNA by polymerase chain reaction assay (PCR) and for antibodies to c100-3, C22-3, c33c and 5-1-1 by the immunoblot, RIBA II test. The two chimpanzees showed different patterns in the sequential and temporal appearance of HCV-RNA and of antibodies to the different HCV epitopes. HCV-specific genomic sequences were detected in serum within one week after infusion in one animal and 9 weeks in the other chimp. Elevations in ALT values were observed 3 weeks subsequent to infusion in both animals.