SIMULTANEOUS DETECTION OF HCV AND HIV-1 SPECIFIC SEQUENCES

目前，丙型肝炎病毒（HCV）和人类 免疫缺陷病毒1型（HIV-1）是基于存在的 血清或血浆中的病毒抗体（Choo，Q.L.，例如，科学、 244：259-262，1989; Petricciani，J.，国际医学年鉴，103，726-729，1985）。 聚合酶链反应（PCR）是一种体外引物导向的方法， 病毒靶序列的酶促扩增，已被用于检测 HCV和HIV-1特异性序列在有症状的 和无症状受试者。PCR的灵敏度允许检测 血清或血浆中极少量的病毒基因组序列， 感染的人。如果临床样本的体积有限， 可用，一种测试，可以同时检测特定的 在单一步骤中获得HCV和HIV-1的病毒序列将是有利的。 基于HCV的核苷酸序列（Choo，Q.L.，例如，科学、 244：259-262，-1989）和HIV-1 gag区，我们已经开发了一种特异性的 和敏感的cDNA/聚合酶链反应检测， 检测个体血清中的HCV和HIV-1特异性序列。 这项研究表明，HCV和HIV-1的多个引物对可以被检测到。 在单个cDNA扩增反应中同时使用以鉴定 两种不同病毒的特定序列

At the present time, screening tests for hepatitis C virus (HCV) and Human Immunodeficiency virus Type 1 (HIV-1) are based on the presence of antibodies to the virus in sera or plasma (Choo, Q.L., et al., Science, 244:259-262, 1989; Petricciani, J., Ann. Inter.Med., 103, 726-729, 1985). The polymerase chain reaction (PCR), an in vitro method for primer-directed enzymatic amplification of viral target sequences, has been used to detect both HCV and HIV-1 specific sequences in sera and plasma of symptomatic and asymptomatic subjects. The sensitivity of PCR allows the detection of extremely small quantities of viral genomic sequences in serum or plasma of infected individuals. Where limited volumes of clinical samples are available, a test that would allow the simultaneous detection of specific viral sequences of HCV and HIV-1 in a single step would be advantageous. Based on the nucleotide sequences of HCV (Choo, Q.L., et al., Science, 244:259-262,-1989) and the HIV-1 gag region, we have developed a specific and sensitive cDNA/polymerase chain reaction assay for the simultaneous detection of HCV and HIV-1 specific sequences in the sera of individuals. This study demonstrates that multiple primer pairs of HCV and HIV-1 can be used simultaneously in a single cDNA amplification reaction to identify specific sequences from two different viruses.