SIMULTANEOUS DETECTION OF HCV AND HIV-1 SPECIFIC SEQUENCES

同时检测 HCV 和 HIV-1 特异性序列

• 批准号: 3811114
• 负责人: S NEDJAR
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3811114
• 关键词: AIDS; AIDS /HIV test; HIV infections; biological products; blood transfusion; communicable disease control; communicable disease diagnosis; communicable disease transmission; diagnosis design /evaluation; hepatitis C virus; human immunodeficiency virus 1; human tissue; nucleic acid sequence; polymerase chain reaction; virus DNA; virus classification

At the present time, screening tests for hepatitis C virus (HCV) and Human Immunodeficiency virus Type 1 (HIV-1) are based on the presence of antibodies to the virus in sera or plasma (Choo, Q.L., et al., Science, 244:259-262, 1989; Petricciani, J., Ann. Inter.Med., 103, 726-729, 1985). The polymerase chain reaction (PCR), an in vitro method for primer-directed enzymatic amplification of viral target sequences, has been used to detect both HCV and HIV-1 specific sequences in sera and plasma of symptomatic and asymptomatic subjects. The sensitivity of PCR allows the detection of extremely small quantities of viral genomic sequences in serum or plasma of infected individuals. Where limited volumes of clinical samples are available, a test that would allow the simultaneous detection of specific viral sequences of HCV and HIV-1 in a single step would be advantageous. Based on the nucleotide sequences of HCV (Choo, Q.L., et al., Science, 244:259-262,-1989) and the HIV-1 gag region, we have developed a specific and sensitive cDNA/polymerase chain reaction assay for the simultaneous detection of HCV and HIV-1 specific sequences in the sera of individuals. This study demonstrates that multiple primer pairs of HCV and HIV-1 can be used simultaneously in a single cDNA amplification reaction to identify specific sequences from two different viruses.

目前，丙型肝炎病毒(丙型肝炎病毒)和人类 免疫缺陷病毒1型(HIV-1)是基于 血清或血浆中的病毒抗体(Choo，Q.L.等，Science， 244：259-262,1989；Petricciani，J.，Ann。国际医学，103,726-729,1985)。 聚合酶链式反应(PCR)--一种引物定向的体外检测方法 病毒靶序列的酶扩增已被用于检测 有症状患者血清和血浆中丙型肝炎病毒和HIV-1的特异性序列 和无症状的受试者。聚合酶链式反应的敏感性使得可以检测到 极少量的血清或血浆中的病毒基因组序列 被感染的人。临床样本数量有限的地方 可用，该测试将允许同时检测特定的 在单一步骤中获得丙型肝炎病毒和艾滋病毒-1的病毒序列将是有利的。 基于丙型肝炎病毒的核苷酸序列(Choo，Q.L.等，Science， 244：259-262，-1989)和HIV-1 Gag区，我们已经开发出一种特异的 同时建立了敏感的cDNA/聚合酶链式反应检测方法 检测个体血清中丙型肝炎病毒和HIV-1的特异性序列。 这项研究表明，丙型肝炎病毒和HIV-1的多对引物可以 同时用于单一的cDNA扩增反应以鉴定 来自两种不同病毒的特定序列。