ESTABLISHMENT OF HCV CONTAINING CELL CULTURE

含HCV细胞培养物的建立

• 批准号: 3770427
• 负责人: S NEDJAR
• 金额: --
• 依托单位: BUREAU OF HEALTH PLANNING AND RESOURCES DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3770427
• 关键词: hepatitis C virus; human tissue; liver cells; method development; tissue /cell culture

The genome of hepatitis C virus (HCV), the etiologic agent of most post- transfusion non-A, non-B cases, is a positive stranded RNA virus. The susceptibility of chimpanzees has allowed collection of serial blood and liver biopsies from all stages of infection, yet no complete virions have been convincingly isolated. A likely reason for the failure to demonstrate these agent(s) or to detect virus-like antigens is the very limited degree of viral replication in the infected host, as suggested by low infectivity titer of most plasma. We anticipate that the development of a primary culture of liver cells might prove susceptible.

丙型肝炎病毒（HCV）是大多数慢性乙型肝炎患者的病原体， 输血非甲、非乙病例，是一种正链RNA病毒。 的 黑猩猩的易感性允许连续收集血液， 所有感染阶段的肝活检，但没有完整的病毒体， 令人信服的孤立。 一个可能的原因是没有证明 这些试剂或检测病毒样抗原是非常有限的程度 病毒复制在受感染的宿主，如低传染性所示 大多数血浆的滴度。我们预计，发展一个主要的 肝细胞的培养可能是敏感的。