A COMPARISON OF STRAINS OF E. COLI TO PRODUCE SEPTIC SHOCK IN DOGS

虽然活细菌通常会引起感染，但它们的相对作用 细菌毒素与活细菌相比，产生的发病率和死亡率 尚不清楚，细菌毒素是否是重要因素，针对 这些毒素可能是必需的。即使没有细菌存活或生长， 这些预先形成的毒素可能在确定发病率和 死亡率。针对这些毒素的治疗可能是对 抗生素治疗。 在犬模型中使用两种大肠杆菌，我们设计了一项研究，以确定 无论是生物毒力因素还是细菌毒素更重要 会导致败血症休克。血液动力学休克，血培养， 内毒素水平和存活率是在两种生物活着的情况下连续进行的， 这两种生物都被杀死了，并从这些生物中提取了纯化的内毒素。 这项研究花了大约4个人的时间。初步结果 已在美国临床医学联合会上发表 研究和重症护理医学学会。这场演讲赢了 1990年的海报奖。人们对这些数据非常感兴趣，因为 确定导致感染发病率的因素将指导 感染性休克儿童和成人未来治疗的试验。

While live bacteria commonly cause infection, the relative role of bacterial toxins versus live bacteria to produce morbidity and mortality is not known, if bacterial toxins are important factors, therapies directed at these toxins may be required. Even if no bacteria remain alive or grow, these preformed toxins may be important in determining morbidity and mortality. Therapies directed at these toxin may be important additions to antibiotic therapy. Using two E. coli in the canine model, we designed a study to determine whether organism virulence factors or bacterial toxins were more important in producing septic shock. Measures of hemodynamic shock, blood cultures, endotoxin levels and survival were done serially with both organisms live, both organisms killed, and with purified endotoxin from these organisms. Approximately 4 man years have gone into this study. Preliminary results from this study have been presented at the American Federation of Clinical Research and the Society of Critical Care Medicine. This presentation won a poster award in 1990. There has been much interest in these data as determination of the factors that produce morbidity in infection will guide trials for future therapies of children and adults with septic shock.