A COMPARISON OF TWO STRAINS OF E COLI TO PRODUCE SEPTIC SHOCK IN DOGS

虽然活细菌通常会引起感染，但它们的相对作用 细菌毒素与活细菌的致残率和死亡率 如果细菌毒素是重要的因素，治疗方法 可能需要针对这些毒素。即使没有细菌存活 或生长，这些预先形成的毒素可能在确定发病率方面很重要 和死亡率。针对这些毒素的治疗可能很重要 抗生素治疗的补充。 利用两株无毒大肠杆菌(O86：H8)和一株强毒大肠杆菌 在犬类模型中，我们设计了一项研究，以确定 生物体毒力因子或细菌毒素在 产生败血症休克。血液动力学休克，血培养， 内毒素水平和存活率是在两种生物活着的情况下连续进行的， 这两种生物都被杀死了，并从这些生物中提取了纯化的内毒素。 这项研究花了大约4个人的时间。初步结果 已在美国医学会联合会上发表。 临床研究和重症护理医学学会。这些数据 暗示的毒力因素比执行的要重要得多 内毒素与内毒素血症的关系 为了进一步解决这个问题，我们正在与LCI的汤姆·鲁索合作， NIAID和最近在布法罗大学研究 内毒素作为同一菌株中的毒力因子。我们是 使用基因中断技术在大肠杆菌菌株中生产 内毒素O型侧链缺陷(另一个致病因素)。 这将进一步探讨内毒素在感染性休克中的相对作用。 目前正在做很多工作来针对人类败血症的这种病原体。 令人震惊。随着这些数据的确定，人们对这些数据非常感兴趣 导致感染发病率的因素将指导未来的试验 儿童和成人感染性休克的治疗。

While live bacteria commonly cause infection, the relative role of bacterial toxins versus live bacteria to produce morbidity and mortality is not known, If bacterial toxins are important factors, therapies directed at these toxins may be required. Even if no bacteria remain alive or grow, these preformed toxins may be important in determining morbidity and mortality. Therapies directed at these toxins may be important additions to antibiotic therapy. Using two E. coli nonvirulent E. coli (O86:H8) and a virulent E. coli (06:41 :K2) in the canine model, we designed a study to determine whether organism virulence factors or bacterial toxins were more important in producing septic shock. Measures of hemodynamic shock, blood cultures, endotoxin levels and survival were done serially with both organisms live, both organisms killed, and with purified endotoxin from these organisms. Approximately 4 man years have gone into this study. Preliminary results from these studies have been presented at the American Federation of Clinical Research and the Society of Critical Care Medicine. These data suggested virulence factors are much more important than performed endotoxins on development of endotoxemia. To further address this issue we are collaborating with Tom Russo of LCI, NIAID and more recently the University of Buffalo to examine the role of endotoxin as a virulence factor within the same strain of E. coli. We are using a gene disruption technique to produce in an E. coli strain with a deficiency in endotoxin o type side chains (another virulence factor). This will further explore the relative role of endotoxin in septic shock. Much work is presently being done to target this pathogen in human septic shock. There has been much interest in these data as determination of the factors that produce morbidity in infection will guide trials for future therapies of children and adults with septic shock.