MOLECULAR ANALYSIS OF MAMMALIAN ENDOGENOUS RETROVIRAL SEQUENCES

非洲猴子天生携带猴免疫缺陷病毒(SIV)， 人类免疫缺陷病毒-2(HIV-2)的潜在前体。 亚洲猴子可以通过密切接触从非洲猴子那里感染SIV 在被囚禁期间。此外，从黑猩猩身上分离出的SIV 已被证明与人类致病因子HIV-1密切相关 艾滋病。由于SIV和HIV之间的这种进化关系， 关于猴子传播病毒的问题引起了极大的关注 通过在猴子体内生长的生物制品(如一些疫苗)对人类- 派生的细胞。因此，我们开始分析各种猴子的DNA 对于可能潜在的内源性SIV/HIV相关序列 产生一种传染性病毒。进行了巢式聚合酶链式反应分析。 从加勒比海非洲绿中提取的高分子量DNA 猴，来自亚洲猕猴，包括食蟹猴、猪尾猴和恒河猴 和来自人类的。聚合酶链式反应是基于高度保守的序列。 已知的SIV和HIV的POL区域。聚合酶链式反应的琼脂糖凝胶分析 产物显示了来自所有物种的扩增片段。我们是 目前正在分子克隆这些片段，并将获得它们的 核苷酸序列来确定这些基因的进化关联性 扩增出已知感染性SIV和HIV的内源序列。

African monkeys naturally harbor simian immunodeficiency viruses (SIVs), the potential progenitors of human immunodeficiency virus-2 (HIV-2). Asian monkeys can acquire SIV from African monkeys through close contact while in captivity. In addition, an SIV isolated from a chimpanzee has been shown to be closely related to HIV-1, the causative agent of human AIDS. Due to this evolutionary relationship between SIVs and HIVs, there is great concern regarding transmission of the virus from monkey to man via biological products (such as some vaccines) grown in monkey- derived cells. We thus undertook to analyze a variety of monkey DNAs for endogenous SIV/HIV-related sequences which might potentially generate an infectious virus. Nested PCR analysis were carried out on high-molecular-weight DNAs isolated from a Carribbean African green monkey, from Asian monkeys including cynomolgous, pig-tailed, and rhesus and from man. PCR primers were based upon highly conserved sequences in the pol regions of known SIVs and HIVs. Agarose gel analysis of the PCR products indicated amplified fragments from all species. We are currently molecularly cloning these fragments and will obtain their nucleotide sequences to determine the evolutionary relatedness of these amplified endogenous sequences to known infectious SIVs and HIVs.