MOLECULAR ANALYSIS OF MAMMALIAN ENDOGENOUS RETROVIRAL SEQUENCES

哺乳动物内源性逆转录病毒序列的分子分析

• 批准号: 3792555
• 负责人: A S KHAN
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3792555
• 关键词: Cercopithecidae; DNA; Macaca fascicularis; Macaca mulatta; Primates; Retroviridae; biochemical evolution; gel electrophoresis; human immunodeficiency virus 1; molecular cloning; natural gene amplification; nucleic acid sequence; polymerase chain reaction; simian immunodeficiency virus; species difference; virus classification

African monkeys naturally harbor simian immunodeficiency viruses (SIVs), the potential progenitors of human immunodeficiency virus-2 (HIV-2). Asian monkeys can acquire SIV from African monkeys through close contact while in captivity. In addition, an SIV isolated from a chimpanzee has been shown to be closely related to HIV-1, the causative agent of human AIDS. Due to this evolutionary relationship between SIVs and HIVs, there is great concern regarding transmission of the virus from monkey to man via biological products (such as some vaccines) grown in monkey- derived cells. We thus undertook to analyze a variety of monkey DNAs for endogenous SIV/HIV-related sequences which might potentially generate an infectious virus. Nested PCR analysis were carried out on high-molecular-weight DNAs isolated from a Carribbean African green monkey, from Asian monkeys including cynomolgous, pig-tailed, and rhesus and from man. PCR primers were based upon highly conserved sequences in the pol regions of known SIVs and HIVs. Agarose gel analysis of the PCR products indicated amplified fragments from all species. We are currently molecularly cloning these fragments and will obtain their nucleotide sequences to determine the evolutionary relatedness of these amplified endogenous sequences to known infectious SIVs and HIVs.

非洲猴子天然携带猿免疫缺陷病毒（SIV）， 人类免疫缺陷病毒-2（HIV-2）的潜在祖细胞。 亚洲猴子可以通过密切接触从非洲猴子那里获得SIV 在囚禁期间 此外，从黑猩猩身上分离出的SIV 已被证明与HIV-1密切相关，HIV-1是人类感染的病原体， 艾滋病 由于SIV和HIV之间的这种进化关系， 人们非常担心病毒从猴子传播 通过在猴子身上生长的生物产品（如一些疫苗）向人类传播- 衍生细胞 因此，我们着手分析各种猴子的DNA， 对于内源性SIV/HIV相关序列， 产生传染性病毒。 巢式PCR分析在 从加勒比海非洲绿色分离的高分子量DNA 猴，来自亚洲猴，包括食蟹猴、猪尾猴和恒河猴 PCR引物是基于人的高度保守序列， 已知SIV和HIV的pol区域。 PCR的琼脂糖凝胶分析 结果显示所有物种的扩增片段。 我们 目前正在对这些片段进行分子克隆， 核苷酸序列，以确定这些进化相关性 已知感染性SIV和HIV的扩增内源性序列。