DEVELOPMENT OF A MONKEY MODEL FOR TESTING OF ANTIVIRAL AGENTS AGAINST HIV-1
DEVELOPMENT OF A MONKEY MODEL FOR TESTING OF ANTIVIRAL AGENTS AGAINST HIV-1
批准号:
3770322
负责人:
A S KHAN
金额:
$0.0万
依托单位:
--
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
关键词:
AIDS therapy Macaca nemestrina antiviral agents blood chemistry cytotoxic T lymphocyte disease /disorder model drug screening /evaluation helper T lymphocyte human immunodeficiency virus 1 leukocyte count model design /development nonhuman therapy evaluation polymerase chain reaction recombinant DNA suppressor T lymphocyte virus DNA virus envelope western blottings
中文摘要
人类免疫缺陷病毒(HIV-1)会导致人类感染艾滋病。这个
HIV-1感染和疾病动物模型系统的建立
对艾滋病疫苗研究和干预至关重要。目前,唯一的
能够感染HIV-1的动物物种是黑猩猩;然而,
疾病就产生了。由于养育一只黑猩猩的巨大成本
事实上,这不是一种疾病模型,人们正在寻找一种更好的模型
根据华盛顿灵长类动物研究中心的一份报告,研究
对猪尾猴的HIV-1感染情况进行了调查。四
已经给猴子接种了两种不同的HIV-1毒株
它们的包膜区域:两只猴子接受了LAV,它的包膜与
实验室对HIV-1 IIIB毒株进行了改造;两只猴子获得了
原代分离株(<;4代),其包膜区域与MN相似
毒株,它代表了北美艾滋病毒-1分离株的大多数。这是
将使用血液样本分析猴子的传染性如下
接种后每两周收集一次:将为病毒准备PBL
分离;取血浆,用Western印迹法检测抗体
分析:将从外周血淋巴细胞中提取DNA,用于用聚合酶链式反应分析病毒DNA;
血液将进行CD4和CD8的表型分析;血清化学也将进行
已执行。
此外,正在构建重组HIV-1DNA以产生
病毒对猴子细胞的趋向性增强。这样的病毒也将成为
在体内测试其在开发猴子系统中的潜在用途
研究HIV-1诱导的艾滋病。
英文摘要
The human immunodeficiency virus (HIV-1) causes AIDS in man. The
development of an animal model system for infection and disease by HIV-1 is
essential for AIDS vaccine studies and intervention. Currently, the only
animal species which can be infected by HIV-1 is the chimpanzee; however, n
disease is produced. Due to the enormous cost of maintaining a chimpanzee
and the fact that this cannot be a disease model, a better model was sought
Based upon a report from the Washington Primate Research Center, studies
were undertaken to investigate HIV-1 infection in pig-tail monkeys. Four
monkeys have been inoculated with two isolates of HIV-1 which differ in
their env regions: two monkeys received LAV, which is similar in its env to
the laboratory adapted strain, HIV-1 IIIB; and two monkeys received a
primary isolate (<4 passages) which is similar in its env region to the MN
strain, which represents the majority of North American HIV-1 isolates. Th
monkeys will be analyzed for infectivity as follows using blood samples
collected every two weeks post-inoculation: PBLs will be prepared for viru
isolation; plasma will be obtained for antibody detection by Western blot
analysis; DNAs will be prepared from PBLs for analysis of viral DNA by PCR;
blood will be phenotyped for CD4 and CD8; serum chemistry also will be
performed.
Additionally, recombinant HIV-1 DNAs are being constructed to generate a
virus with an increased tropism for monkey cells. Such a virus will also b
tested in vivo for its potential use in developing the monkey system for
studying HIV-1 induced AIDS.
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STRUCTURAL AND FUNCTIONAL STUDIES OF MAMMALIAN ENDOGENOUS RETROVIRAL SEQUENCES
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批准号:3818206
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A S KHAN
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依托单位:
STRUCTURAL AND FUNCTIONAL STUDIES OF MAMMALIAN ENDOGENOUS RETROVIRAL SEQUENCES
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批准号:3809632
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A S KHAN
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依托单位:
STUDY OF A MURINE RETROVIRUS FROM A PACKAGING CELL LINE USED IN GENE THERAPY
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批准号:3770323
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A S KHAN
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依托单位:--
STRUCTURAL AND FUNCTIONAL ANALYSIS OF ENDOGENOUS PROVIRUSES OF MICE
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批准号:3822045
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A S KHAN
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依托单位:
DEVELOPMENT OF A MONKEY MODEL FOR TESTING OF ANTIVIRAL AGENTS AGAINST HIV-1
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批准号:5200716
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A S KHAN
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依托单位:--
DEVELOPMENT OF A MONKEY MODEL FOR TESTING OF ANTIVIRAL AGENTS AGAINST HIV-1
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批准号:3748151
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A S KHAN
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依托单位:--
STUDY OF A MURINE RETROVIRUS FROM A PACKAGING CELL LINE USED IN GENE THERAPY
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批准号:3748152
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A S KHAN
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依托单位:--
MOLECULAR ANALYSIS OF MAMMALIAN ENDOGENOUS RETROVIRAL SEQUENCES
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批准号:3792555
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A S KHAN
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依托单位:--
STRUCTURAL AND FUNCTIONAL STUDIES OF MAMMALIAN ENDOGENOUS RETROVIRAL SEQUENCES
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批准号:3790737
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A S KHAN
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依托单位:
MOLECULAR BIOLOGY AND BIOCHEMICAL STRUCTRUE OF ENDOGENOUS PROVIRUSES OF MICE
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批准号:4688492
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A S KHAN
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依托单位:
STRUCTURAL AND FUNCTIONAL ANALYSIS OF ENDOGENOUS PROVIRUSES OF MICE
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批准号:3960567
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A S KHAN
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依托单位:
STUDY OF A MURINE RETROVIRUS FROM A PACKAGING CELL LINE USED IN GENE THERAPY
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批准号:5200717
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A S KHAN
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依托单位:--
MONKEY MODEL FOR AIDS
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批准号:3792554
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A S KHAN
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依托单位:--
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