DEVELOPMENT OF A MONKEY MODEL FOR TESTING OF ANTIVIRAL AGENTS AGAINST HIV-1

人类免疫缺陷病毒(HIV-1)会导致人类感染艾滋病。这个 HIV-1感染和疾病动物模型系统的建立 对艾滋病疫苗研究和干预至关重要。目前，唯一的 能够感染HIV-1的动物物种是黑猩猩；然而， 疾病就产生了。由于养育一只黑猩猩的巨大成本 事实上，这不是一种疾病模型，人们正在寻找一种更好的模型 根据华盛顿灵长类动物研究中心的一份报告，研究 对猪尾猴的HIV-1感染情况进行了调查。四 已经给猴子接种了两种不同的HIV-1毒株 它们的包膜区域：两只猴子接受了LAV，它的包膜与 实验室对HIV-1 IIIB毒株进行了改造；两只猴子获得了 原代分离株(&lt；4代)，其包膜区域与MN相似 毒株，它代表了北美艾滋病毒-1分离株的大多数。这是 将使用血液样本分析猴子的传染性如下 接种后每两周收集一次：将为病毒准备PBL 分离；取血浆，用Western印迹法检测抗体 分析：将从外周血淋巴细胞中提取DNA，用于用聚合酶链式反应分析病毒DNA； 血液将进行CD4和CD8的表型分析；血清化学也将进行 已执行。 此外，正在构建重组HIV-1DNA以产生 病毒对猴子细胞的趋向性增强。这样的病毒也将成为 在体内测试其在开发猴子系统中的潜在用途 研究HIV-1诱导的艾滋病。

The human immunodeficiency virus (HIV-1) causes AIDS in man. The development of an animal model system for infection and disease by HIV-1 is essential for AIDS vaccine studies and intervention. Currently, the only animal species which can be infected by HIV-1 is the chimpanzee; however, n disease is produced. Due to the enormous cost of maintaining a chimpanzee and the fact that this cannot be a disease model, a better model was sought Based upon a report from the Washington Primate Research Center, studies were undertaken to investigate HIV-1 infection in pig-tail monkeys. Four monkeys have been inoculated with two isolates of HIV-1 which differ in their env regions: two monkeys received LAV, which is similar in its env to the laboratory adapted strain, HIV-1 IIIB; and two monkeys received a primary isolate (<4 passages) which is similar in its env region to the MN strain, which represents the majority of North American HIV-1 isolates. Th monkeys will be analyzed for infectivity as follows using blood samples collected every two weeks post-inoculation: PBLs will be prepared for viru isolation; plasma will be obtained for antibody detection by Western blot analysis; DNAs will be prepared from PBLs for analysis of viral DNA by PCR; blood will be phenotyped for CD4 and CD8; serum chemistry also will be performed. Additionally, recombinant HIV-1 DNAs are being constructed to generate a virus with an increased tropism for monkey cells. Such a virus will also b tested in vivo for its potential use in developing the monkey system for studying HIV-1 induced AIDS.