ENHANCEMENT OF ICAM-1 SENSITIZES TUMOR CELLS TO MONOCYTE-MEDIATED LYSIS

ICAM-1 的增强使肿瘤细胞对单核细胞介导的裂解敏感

• 批准号: 3804743
• 负责人: D S WEBB
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3804743
• 关键词: biological signal transduction; cell adhesion molecules; cell mediated cytotoxicity; cellular immunity; cytokine; cytolysis; human tissue; immunoregulation; leukocyte adhesion molecules; monocyte; neoplasm /cancer immunology; neoplastic cell culture for noncancer research; receptor expression; tissue /cell culture; tumor antigens; tumor necrosis factor alpha

We have previously found that an increase in ICAM-1 expression on two different tumor cell lines resulted in an increased sensitivity of these cells to lysis by human monocytes. Evidence was presented that the increase in vulnerability to lysis was a direct result of the enhanced expression of ICAM-1. The interactions of LFA-1 on human monocytes to ICAM-1 on tumor cells was found to be necessary for lysis to occur. The results of this work were published in The Journal of Immunology, 146:3682-3686, 1991. In the course of this work, we found that the actions of the cytokines on the tumor cells accounts for only part of the overall enhancement of monocyte-mediated cytotoxicity induced by certain cytokines. The exposure of monocytes alone to some cytokines results in an alteration of the LFA-1/ICAM-1 interactions. There are several mechanisms by which exposure of monocytes to cytokines may alter the LFA- 1/FCAM-1 interactions. We have ruled out the possibility that cytokine treatment of monocytes results in increased LFA-1 expression. However, we are currently conducting experiments to determine if the affinity of the LFA-1/ICAM-1 interaction is altered by cytokine treatment, or if cytokine pretreated monocytes release TNF or IL-1 upon contact with tumor cells.

我们之前已经发现两个细胞的ICAM-1表达增加