INTERLEUKIN 1 RELEASE BY HUMAN MONOCYTES

在这项工作的过程中，我们发现人类的前处理 单核细胞经M-CSF刺激后IL-1的释放受到抑制 脂多糖刺激人单核细胞。Northern杂交分析表明， M-CSF不改变内毒素诱导的IL-1信息传递。此外, 使用结合PRO和PRO的抗体进行免疫沉淀 和IL-1的处理形式，表明该消息被翻译成 细胞内IL-1。然而，似乎细胞内的蛋白质并不是 有效地从细胞中释放出来。我们计划利用这些发现来 检测与人单核细胞释放IL-1有关的事件 通过免疫组织化学染色确定是否所有单核细胞 用内毒素刺激产生IL-1，或者只有一小部分人 单核细胞能够产生IL-1。

During the course of this work, we have found that pretreatment of human monocytes with M-CSF results in an inhibition of the release of IL-1 from LPS stimulated human monocytes. Northern blot analysis revealed that M-CSF does not alter the LPS induction of IL-1 message. In addition, immunoprecipitations performed using an antibody which binds both the pro and processed forms of IL-1, indicate that the message is translated into intracellular IL-1. However, it appears the intracellular protein is not effectively released from the cells. We plan to use these findings to examine the events involved in IL-1 release from human monocytes and to determine, by use of immunohistochemical staining whether all monocytes stimulated with LPS produce IL-1 or whether only a subpopulation of monocytes is capable of IL-1 production.