INTERLEUKIN 1 RELEASE BY HUMAN MONOCYTES

人单核细胞释放白细胞介素 1

• 批准号: 3792489
• 负责人: D S WEBB
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3792489
• 关键词: colony stimulating factor; human tissue; immunocytochemistry; immunoprecipitation; interleukin 1; leukocyte activation /transformation; lipopolysaccharides; monocyte; northern blottings; protein biosynthesis

During the course of this work, we have found that pretreatment of human monocytes with M-CSF results in an inhibition of the release of IL-1 from LPS stimulated human monocytes. Northern blot analysis revealed that M-CSF does not alter the LPS induction of IL-1 message. In addition, immunoprecipitations performed using an antibody which binds both the pro and processed forms of IL-1, indicate that the message is translated into intracellular IL-1. However, it appears the intracellular protein is not effectively released from the cells. We plan to use these findings to examine the events involved in IL-1 release from human monocytes and to determine, by use of immunohistochemical staining whether all monocytes stimulated with LPS produce IL-1 or whether only a subpopulation of monocytes is capable of IL-1 production.

在这项工作的过程中，我们发现人类的预处理