EFFECT OF CYTOKINES IN HOST DEFENSE AND INFLAMMATION

细胞因子在宿主防御和炎症中的作用

• 批准号: 3803217
• 负责人: J I GALLIN
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3803217
• 关键词: blister; body fluids; cell migration; child (0-11); colony stimulating factor; complement; cytokine; human subject; inflammation; interferons; interleukin 1; interleukin 4; leukotrienes; monocyte; neutrophil; protein biosynthesis

The purpose of this project was to catalogue the kinetics of the appearance of cytokines and other mediators of inflammation in an experimental inflammatory response in man. For this study blisters were raised on the forearms of human subjects using a suction blister device. One hour after the blister was raised the blister fluid was evaluated for cells and mediators. The floor of the blister was scrapped for assessment of cell content. A lucite template containing a 1 ml chamber was placed over the blister floor and then filled with 70% autologous serum and the accumulation of cells and mediators into the blister fluid monitored over time. The data indicated that neutrophils increased in the blister fluid throughout 24 hours of study with less than 5% of the cells being mononuclear cells under these conditions. Interestingly, over the first 5 h the floor of the blister contained about 40% mononuclear cells. Neat blister fluid contained small but significantly greater amounts of C5a, LTB4, I1-4, Il-8 and Gamma-interferon compared with simultaneously obtained peripheral blood plasma. These mediators continued to increase with Gamma-interferon peaking at about 6 hours and C5a, LTB4 peaking at 8-12 hours and Il-4 and Il-8 peaking at 24 hours. The following mediators were not observed before 8 hours and then continued to increase through 24 hours: TNF-Alpha, Il-1Beta, and GM-CSF. Il-1Alpha and Il-2 were not detected in the blister fluid. Thus, different cytokines appear in this experimental inflammatory focus with different kinetics and the source and regulation of the appearance of these mediators now needs to be studied.

这个项目的目的是编目的动力学 细胞因子和其他炎症介质的出现， 在这项研究中， 使用抽吸泡罩装置在人类受试者的前臂上升起。 在水疱升起后1小时，评价水疱液 for cells细胞and mediators介质.泡罩的底部被废弃， 评估细胞含量。有机玻璃模板，包含1 ml腔室 放置在泡罩底板上，然后填充70%自体 血清以及细胞和介质积聚到水疱液中 随着时间的推移进行监测。数据表明，中性粒细胞增加， 在整个24小时的研究中， 单核细胞在这些条件下。有趣的是， 在最初的5小时内，泡罩底部含有约40%的 单核细胞纯水疱液中含有少量但显著 C5 a、LTB 4、I1-4、Il-8和γ-干扰素的量更高， 同时获得外周血血浆。这些介质 持续增加，γ-干扰素在约6小时达到峰值， C5 a、LTB 4在8-12小时达到峰值，IL-4和IL-8在24小时达到峰值。 在8小时之前未观察到以下介质， 持续增加至24小时：TNF-α、IL-1 β和GM-CSF。 在水疱液中未检测到IL-1 α和IL-2。因此，在本发明中， 不同的细胞因子出现在这个实验性炎症病灶中， 不同的动力学和来源和调节的外观 现在需要对这些调解人进行研究。