NEUTROPHIL SUBPOPULATIONS

人类中性粒细胞异质性的提出是基于 差异趋化反应性和结果的基础上，在我们的 显示Fc受体介导的IgG玫瑰花结差异的实验室 包被的红细胞 检查功能反应性的异质性 使用荧光激活细胞分选仪评估功能 中性粒细胞群对化学引诱物的反应性 通常由E. coli，fmet-leu-phe（fMLP）。 异质性 显示了fMLP的营养结合。 大多数中性粒细胞 最初结合是与高亲和力受体，随后出现 一种低亲和力受体，很容易被额外的 fMLP。 具有这两类受体的细胞被证明是 其反应是膜电位去极化， 硝基蓝四唑染料响应于fMLP。 剩余细胞 （10-20%）仅表现出对fMLP的高亲和力受体， FMET-Leu-Phe激活的证据。 我们开发了一种IgG单克隆抗体 抗体（31 D8），与被激活的中性粒细胞强烈结合 开发了fMLP。 31 D8抗原首先出现在髓细胞阶段 的成熟。 我们使用31 D8研究慢性粒细胞性白血病患者， 白血病（CML），一种克隆性疾病。 5例患者无31 D8阳性细胞 检出 这5例患者在初始治疗时缓解， 研究，随后进展到加速期或急变期。 在 CML 31 D8抗原表达缺失可能是CML的早期标志物， CML的进展。 早期检测将是有益的，因为骨髓 早期进行移植似乎对CML最有效。 我们 也表明新生儿脐带血中性粒细胞减少， 31 D8阳性中性粒细胞数量。

Human neutrophil heterogeneity has been suggested on the basis of differences chemotactic responsiveness and on the basis of results in our laboratory showing differences in Fc receptor mediated rosetting of IgG coated red cells. Heterogeneity of functional responsiveness was examined using the fluorescent activated cell sorter to assess functional responsiveness of neutrophil populations stimulated with a chemoattractant normally produced by E. coli, fmet-leu-phe (fMLP). Heterogeneity of enutrophil binding of fMLP was shown. In the majority of neutrophils initial binding was to a high affinity receptor with subsequent appearance of a low affinity receptor that was readily displaced with additional fMLP. The cells with the two classes of receptors proved to be the ones that responded with depolarization of membrane potential and reduction of nitroblue tetrazolium dye in response to fMLP. The remainder cells (10-20%) only exhibited the high affinity receptor for fMLP and lacked evidence for activation by fmet-leu-phe. We developed an IgG monoclonal antibody (31D8) that binds strongly to neutrophils that are activated with fMLP was developed. The 31D8 antigen first appears at the myelocyte stage of maturation. We used 31D8 to study patients with chronic myelogenous leukemia (CML), a clonal disease. In 5 patients no 31D8 positive cells were detected. These 5 patients, who were in remission at time of initial study, subsequently progressed to accelerated phase or blast crisis. In CML absence of 31D8 antigen expression may be an early marker for progression of CML. Early detection would be useful since bone marrow transplantation appears to be most efficacius in CML when done early. We have also demonstrated that neonate cord blood neutrophils have a decreased number of 31D8 positive neutrophils.