NEUTROPHIL SUBPOPULATIONS

中性粒细胞亚群

• 批准号: 3822020
• 负责人: J I GALLIN
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3822020
• 关键词: biological polymorphism; calcium; cell differentiation; cell population study; chemotaxis; child (0-11); flow cytometry; human subject; immune adherence reaction; immunoglobulin G; ion transport; leukocyte activation /transformation; leukopoiesis; mast cell; monoclonal antibody; myelocyte; myelogenous leukemia; neoplasm /cancer diagnosis; neoplasm /cancer immunology; neutrophil; phagocytes; phagocytosis; protein kinase C

Human neutrophil heterogeneity has been suggested on the basis of differences in chemotactic responsiveness, on the basis of results in our laboratory showing differences in Fc receptor mediated rosetting of IgG coated red cells, and more recently with monoclonal antibodies. Heterogeneity of binding of monoclonal antibodies to human neutrophils was examined using the fluorescent activated cell sorter. We developed an IgG monoclonal antibody, 31D8, that binds strongly to neutrophils that are activated with fMLP and more responsive chemotactically, representing about 95% of normal neutrophils. 31D8 antigen appears at the myelocyte stage of maturation. We used 31D8 to study patients with chronic myelogenous leukemia (CML), a clonal disease and showed that failure to express 31D8 on any mature appearing neutrophils correlated with progression of CML to blast crisis. In other studies we demonstrated that neonate cord blood neutrophils have a decreased number of 31D8 positive neutrophils which correlates with an increase in band forms. Similarly there is a marked decrease in the number of 31D8 cells after severe blunt trauma or after experimental infusion of endotoxin. Band forms and 31D8 weakly reactive cells comprise an overlapping but not identical population. 31D8 expression may be a measure of neutrophil maturation independent of nuclear morphology and may be useful to measure the degree of marrow release of neutrophils. Dr. Segal developed IgG monoclonal antibody, C10, which we have characterized as binding strongly to about 30 to 80% of neutrophils. Percent C10 positive neutrophils in the same individual is constant. Further studies are in progress.

人类中性粒细胞的异质性已被认为是基于 趋化反应的差异，基于 我们实验室的结果显示Fc受体 介导的IgG包被的红细胞的玫瑰花结，以及最近 单克隆抗体。 结合的异质性 用抗人中性粒细胞的单克隆抗体进行检测， 荧光激活细胞分选仪。 我们开发了一种IgG 一种单克隆抗体，31D8，与中性粒细胞强烈结合， 被fMLP激活，并且在化学活性上更敏感， 代表约95%的正常中性粒细胞。 31D8抗原 出现在成熟的髓细胞阶段。 我们使用31D8 慢性粒细胞白血病（CML）是一种克隆性白血病， 疾病，并表明未能表达31 D8上任何成熟的， 中性粒细胞的出现与CML向急变的进展相关 危机 在其他研究中，我们证明新生儿脐带血 中性粒细胞中31D8阳性中性粒细胞数量减少 这与乐队形式的增加有关。 类似地， 在严重的免疫后，31D8细胞的数量明显减少， 钝伤或实验性内毒素输注后。 带 形式和31D8弱反应细胞包括重叠，但 不是相同的人口。 31D8表达可能是一个衡量指标， 中性粒细胞成熟不依赖于核形态， 可用于测量中性粒细胞的骨髓释放程度。 博士Segal开发了IgG单克隆抗体，C10，我们有 其特征在于与约30至80%的 中性粒细胞 C10阳性中性粒细胞百分比 个体是恒定的。 进一步的研究正在进行中。