IDENTIFICATION OF EBV PROTEINS THAT STIMULATE MONOKINE SECRETION

刺激单核因子分泌的 EBV 蛋白的鉴定

• 批准号: 3804745
• 负责人: K CLOUSE-STREBEL
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3804745
• 关键词: Epstein Barr virus; HIV infections; gene expression; glycosylation; human immunodeficiency virus; human tissue; immunoregulation; leukocyte activation /transformation; macrophage; monoclonal antibody; monocyte; monokines; secretion; virus antigen; virus envelope; virus infection mechanism; virus protein; virus receptors; virus replication

We have previously shown that certain inactivated viruses, including strains of Epstein-Barr virus (EBV), can stimulate human monocyte/macrophages (MO) to secrete monokines capable of upregulating human immunodeficiency virus (HIV) expression in chronically infected human cells. Our current objective was to focus on EBV proteins to determine whether natural proteins affinity purified using specific monoclonal antibodies, are responsible for the monocyte stimulation leading to this effect. Monokine secretion has been determined in response to the EBV gp350 envelope protein, the gp125 viral capsid protein, the p50 early antigen (diffuse distribution), and the p17 early antigen (restricted distribution). Both gp350 and gp125 are capable of stimulating the secretion of monokines which upregulate HIV expression, while data using p50 and p17 are relatively inconsistent. Monokine secretion caused by the EBV glycoproteins is not attributable to contaminating endotoxin. This suggests that some, but not all, EBV proteins may indirectly affect the expression of HIV during the course of an HIV infection by stimulating monokine production. Whether this is a receptor-mediated phenomenon or whether glycosylation is involved in this response is currently under investigation.

我们之前已经证明了某些灭活病毒，包括 EB病毒(EBV)株，可刺激人类 单核/巨噬细胞(MO)分泌能够上调的单因子 人类免疫缺陷病毒(HIV)在慢性感染中的表达 人类细胞。我们目前的目标是专注于EBV蛋白来 确定天然蛋白是否使用特定的亲和层析纯化 单抗，负责单核细胞的刺激 导致了这种效果。Monokine的分泌已经在 对EBV gp350囊膜蛋白gp125病毒衣壳的应答 蛋白质、p50早期抗原(弥漫性分布)和p17早期 抗原(受限分布)。Gp350和gp125都能够 刺激单核细胞的分泌，上调艾滋病毒的表达， 而使用P50和P17的数据相对不一致。Monokine EBV糖蛋白引起的分泌物不是由 污染了内毒素。这表明，一些，但不是全部，EBV 蛋白质可能间接影响HIV在感染过程中的表达 通过刺激单核细胞的产生而感染艾滋病毒。无论这是一个 受体介导的现象或糖基化是否参与其中 回应目前正在调查中。