INHIBITION OF HIV ACTIVATION BY SOLUBLE TUMOR NECROSIS FACTOR RECEPTOR
INHIBITION OF HIV ACTIVATION BY SOLUBLE TUMOR NECROSIS FACTOR RECEPTOR
批准号:
3792477
负责人:
K CLOUSE-STREBEL
金额:
$0.0万
依托单位:
--
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
中文摘要
炎性细胞因子,肿瘤坏死因子- α (tnf - α)
英文摘要
The inflammatory cytokine, tumor necrosis factor-alpha (TNF-alpha), has
been shown to stimulate HIV-1 replication in both chronically and acutely
infected T-lymphocytes and monocytes. The activation of the HIV-Long
Terminal Repeat (HIV-LTR) and subsequent increase in virus production is
linked to TNF activation of the cellular transcription factor, NF-kB. We
have tested the ability of two forms of soluble recombinant type 1 (p80)
TNF receptor to inhibit TNF-induced HIV activation in vitro. One form of
the receptor is a monomer containing the entire 234 residues of the
extracellular (ligand-binding) portion of p80. A second form is a
homodimer chimeric protein containing these same residues fused to a
truncated human IgG(l) immunoglobulin chain, and thus resembles a
bivalent antibody without light chains. These recombinant proteins were
tested for their ability to inhibit TNF-alpha-induced expression of HIV-1
in chronically infected human cell lines as determined by viral reverse
transcriptase activity. We also examined the ability of the soluble
receptors to limit the activation of HIV-LTR transcription. The soluble
TNF receptor dimer was most effective at blocking the TNF-alpha-induced
expression in both monocytic and lymphocytic cell lines. The ratio of
receptor to TNF-alpha was critical, with optimal inhibition requiring a
10-fold excess of receptor. Monomeric receptor was less effective at
blocking and, at times, was capable of augmenting the activity of
TNF-alpha. Thus, our data suggests that TNF-alpha-induced HIV-1
expression can be limited in vitro using a dimeric form of the TNF
receptor at specific ratios in excess of TNF-alpha.
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MODULATION OF HIV-1 REPLICATION BY CYTOKINES AND SOLUBLE CYTOKINE RECEPTORS
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批准号:5200743
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项目类别:
-
资助金额:$0.0万
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财政年份:--
-
负责人:K CLOUSE-STREBEL
-
依托单位:--
IDENTIFICATION OF HIV PROTEINS THAT STIMULATE MONOKINE SECRETION
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批准号:3811210
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:K CLOUSE-STREBEL
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依托单位:--
IDENTIFICATION OF EBV PROTEINS THAT STIMULATE MONOKINE SECRETION
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批准号:3792465
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:K CLOUSE-STREBEL
-
依托单位:--
MODULATION OF HIV-1 REPLICATION BY CYTOKINES AND SOLUBLE CYTOKINE RECEPTORS
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批准号:3748181
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:K CLOUSE-STREBEL
-
依托单位:--
IDENTIFICATION OF EBV PROTEINS THAT STIMULATE MONOKINE SECRETION
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批准号:3811211
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:K CLOUSE-STREBEL
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依托单位:--
REGULATION OF CYTOKINE EXPRESSION BY HIV
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批准号:5200742
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:K CLOUSE-STREBEL
-
依托单位:--
IDENTIFICATION OF HIV PROTEINS THAT STIMULATE MONOKINE SECRETION
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批准号:3804744
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:K CLOUSE-STREBEL
-
依托单位:--
IDENTIFICATION OF EBV PROTEINS THAT STIMULATE MONOKINE SECRETION
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批准号:3804745
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:K CLOUSE-STREBEL
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依托单位:--
STIMULATION OF MONOCYTIC ENDOTHELIN-1 PRODUCTION BY HIV-1 GP120
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批准号:3792478
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:K CLOUSE-STREBEL
-
依托单位:--
REGULATION OF CYTOKINE EXPRESSION BY HIV
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批准号:3748180
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:K CLOUSE-STREBEL
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依托单位:--
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