IDENTIFICATION OF EBV PROTEINS THAT STIMULATE MONOKINE SECRETION

刺激单核因子分泌的 EBV 蛋白的鉴定

• 批准号: 3792465
• 负责人: K CLOUSE-STREBEL
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3792465
• 关键词: Epstein Barr virus; human tissue; immunoregulation; leukocyte activation /transformation; macrophage; monoclonal antibody; monocyte; monokines; virus protein

We have previously shown that certain inactivated viruses, including strains of Epstein-Barr virus (EBV), can stimulate human monocyte/macrophages (MO) to secrete monokines capable of upregulating human immunodeficiency virus (HIV) expression in chronically infected human cells. Our current objective was to focus on EBV proteins to determine whether natural proteins affinity purified using specific monoclonal antibodies, are responsible for the monocyte stimulation leading to this effect. Monokine secretion has been determined in response to the EBV gp350 envelope protein, the gpl25 viral capsid protein, the p50 early antigen (diffuse distribution), and the pl7 early antigen (restricted distribution). Both gp350 and gpl25 are capable of stimulating the secretion of monokines which upregulate HIV expression, while data using p50 and pl7 are relatively inconsistent. Monokine secretion caused by the EBV glycoproteins is not attributable to contaminating endotoxin. This suggests that some, but not all, EBV proteins may indirectly affect the expression of HIV during the course of an HIV infection by stimulating monokine production. Whether this is a receptor-mediated phenomenon or whether glycosylation is involved in this response is currently under investigation.

我们以前已经证明，某些灭活病毒，包括 EB病毒（EBV）株，可刺激人类 单核细胞/巨噬细胞（MO）分泌能够上调 人类免疫缺陷病毒（HIV）在慢性感染者中表达 人类细胞。 我们目前的目标是关注EBV蛋白， 确定天然蛋白质是否使用特异性亲和纯化 单克隆抗体负责单核细胞刺激 导致这种效果。 单核细胞因子分泌已被确定， 对EBV gp 350包膜蛋白、gp 125病毒衣壳的应答 蛋白、p50早期抗原（弥散分布）和p17早期抗原（弥散分布）。 抗原（限制性分布）。 gp 350和gp 125都能够 刺激上调HIV表达的单核因子的分泌， 而使用P50和P17的数据相对不一致。 单核因子 由EBV糖蛋白引起的分泌不能归因于 污染内毒素。 这表明，一些，但不是全部，EBV 蛋白质可能间接影响艾滋病毒在感染过程中的表达 通过刺激单核细胞因子的产生来预防HIV感染。 这是否是一个 受体介导的现象或糖基化是否参与其中 目前正在调查答复。