IDENTIFICATION OF EBV PROTEINS THAT STIMULATE MONOKINE SECRETION

刺激单核因子分泌的 EBV 蛋白的鉴定

• 批准号: 3811211
• 负责人: K CLOUSE-STREBEL
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3811211
• 关键词: Epstein Barr virus; HIV infections; affinity chromatography; human immunodeficiency virus; immunoregulation; macrophage; monocyte; monokines; virus antigen; virus envelope; virus infection mechanism; virus protein; virus replication

We have previously shown that certain inactivated viruses, including strains of Epstein-Barr virus (EBV), can stimulate human monocyte/macrophages (MO) to secrete monokines capable of upregulating human immunodeficiency virus (HIV) expression in chronically infected human cells. Our current objective was to focus on EBV proteins to determine whether natural proteins affinity purified using specific monoclonal antibodies, are responsible for the monocyte stimulation leading to this effect. Monokine secretion has been determined in response to the EBV gp350 envelope protein, the gpl25 viral capsid protein, the p5O early antigen (diffuse distribution), and the pl7 early antigen (restricted distribution). Both gp350 and gpl25 are capable of stimulating the secretion of monokines which upregulate HIV expression, while data using p50 and pl7 are relatively inconsistent. Monokine secretion caused by the EBV glycoproteins is not attributable to contaminating endotoxin. This suggests that some, but not all, EBV proteins may indirectly affect the expression of HIV during the course of an HIV infection by stimulating monokine production. Whether this is a receptor-mediated phenomenon or whether glycosylation is involved in this response is currently under investigation.

我们以前已经证明，某些灭活病毒，包括 EB病毒（EBV）株，可刺激人类 单核细胞/巨噬细胞（MO）分泌能够上调 人类免疫缺陷病毒（HIV）在慢性感染者中表达 细胞我们目前的目标是关注EBV蛋白，以确定 是否使用特异性单克隆抗体亲和纯化天然蛋白质 抗体，负责单核细胞刺激导致这一点， 效果已经确定了响应EBV gp 350的单核因子分泌 包膜蛋白、gp 125病毒衣壳蛋白、p50早期抗原 P17早期抗原（限制性） 分配）。gp 350和gp 125都能够刺激细胞的增殖。 分泌的单核因子，上调艾滋病毒的表达，而数据使用 p50和p17相对不一致。单核细胞因子分泌引起的 EBV糖蛋白不能归因于污染的内毒素。这 提示一些但不是全部的EBV蛋白可能间接影响了 在HIV感染过程中，通过刺激 单核因子产生。这是否是受体介导的现象， 糖基化是否参与这种反应目前尚不清楚。 调查