ISOLATION AND CHARACTERIZATION OF A NOVEL H-2 CLASS I GENE

类MHC基因家族中高度分化的成员m1代表一种 原始I类基因的直系后裔，它早于物种形成。 使用一系列重组和同源小鼠品系，该基因具有 被定位到17号染色体端粒到Qa的一个区域。结合 随着对其他不同的I类基因的研究，例如那些编码HMT的基因， M1被用来定义一个新的MHC次区域M。 尽管M1与H-2 I类的其余部分有很大的差异 家族，M1有开放的阅读框架和合法的剪接位点 外显子。M1似乎含有一个功能性启动子，因为引入了 3.4kb含M1的基因组片段导入小鼠L细胞 基因的转录。然而，M1不能被检测到转录 在各种细胞系或成体组织中，没有M1抗原 已被确认身份。M1的表达受一个强大的抑制器控制 位于包含M1基因的16kb基因组片段内的元件。

A highly divergent member of the class I MHC gene family, M1, represents a direct descendant of a primordial class I gene, which antedates speciation. Using a series of recombinant and congenic strains of mice, this gene has been mapped to a region of chromosome 17 telomeric to Qa. In conjunction with studies of other divergent class I genes, such as those encoding HMT, M1 has been used to define a new MHC subregion, M. Despite the extensive divergence of M1 from the rest of the H-2 class I family, M1 has open reading frames and legitimate splice sites in all exons. M1 appears to contain a functional promoter since introduction of a 3.4 kb genomic DNA fragment containing M1 into mouse L cells results in transcription of the gene. Nevertheless, M1 is not detectably transcribed in a variety of cell lines or adult somatic tissues and no M1 antigen has been identified. Expression of M1 is controlled by a strong silencer element located within a 16 kb genomic fragment containing the M1 gene.