ISOLATION AND CHARACTERIZATION OF A NOVEL H-2 CLASS I GENE

新型 H-2 I 类基因的分离和表征

• 批准号: 3813472
• 负责人: D SINGER
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3813472
• 关键词: DNA; biochemical evolution; gene expression; genetic mapping; genetic recombination; genetic strain; genetic transcription; genome; histocompatibility gene; laboratory mouse; membrane proteins; nucleic acid sequence; restriction fragment length polymorphism; transfection

A highly divergent member of the class I MHC gene family, M1, represents a direct descendant of a primordial class I gene, which antedates speciation. Using a series of recombinant and congenic strains of mice, this gene has been mapped to a region of chromosome 17 telomeric to Qa. In conjunction with studies of other divergent class I genes, such as those encoding HMT, M1 has been used to define a new MHC subregion, M. Despite the extensive divergence of M1 from the rest of the H-2 class I family, M1 has open reading frames and legitimate splice sites in all exons. M1 appears to contain a functional promoter since introduction of a 3.4 kb genomic DNA fragment containing M1 into mouse L cells results in transcription of the gene. Nevertheless, M1 is not detectably transcribed in a variety of cell lines or adult somatic tissues and no M1 antigen has been identified. Expression of M1 is controlled by a strong silencer element located within a 16 kb genomic fragment containing the M1 gene.

一类MHC基因家族的一个高度分化的成员，M1，代表一个