PERIODONTAL DISEASE RESEARCH CENTER

牙周疾病研究中心

• 批准号: 3105646
• 负责人: WILLIAM B CLARK
• 金额: $ 70.32万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-01 至 1993-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3105646
• 关键词: dental disorder diagnosis; human subject; oral bacteria; periodontitis; periodontium disorder

The major objectives of this Center are to develop modalities for diagnosis, treatment, and prevention of periodontal diseases. A series of related clinical, microbiological, and immunological studies have been designed to address these objectives. The clinical component is designed to detect early disease activity utilizing highly sensitive detection methods such as an electronic constant-force probe which has been shown to be highly sensitive and reproducible. Microbiologic and immunologic studies are designed to investigate the microbial etiologies of refractory and suppurative periodontal diseases and to define the interrelationship between specific microorganisms and the host's immune response. Clinical measurements will be performed to verify that individual sites are in an actively progressing state of disease prior to therapy and in a state of remission after therapy. The composition of the microbiota and the immune response will be examined longitudinally in each patient to determine the overall effects of different therapeutic regimens. Determination of the effect of different periodontal therapies on the clinical response the host's microbiota, and the immune response, should lead to more rational approaches in the treatment of these diseases. By thoroughly investigating the multiplicity of interrelated factors associated with the periodontal disease process, it hoped that bases for rational prevention and therapy will be established. The objective of the squirrel monkey (Saimiri sciureus) study is to develop an in vivo model for colonization with suspected periodontal pathogens (e.g., B. gingivalis, H. actinomycetemcomitans) and then to examine the effects of immunization on colonization. The specific aims are: (1) establish an association between one or more subgingival bacteria (e.g., B. gingivalis) and disease; (2) define the relationship of immunity to suspected periodontal pathogens; (3) investigate the mechanisms of infection and oral colonization of monkeys with pathogens; (4) identify and characterize the antigens from pathogenic bacteria against which normally infected and immunized monkeys make antibody; and (5) test the effect of immunization on colonization of subgingival plaque with pathogenic bacteria. From these studies, the microbiota involved in disease as well as the humoral immune response to these bacteria will be defined. A model of colonization of subgingival plaque by identified pathogens will be developed. This model will then be used to study the potential of the humoral immune response to modulate the oral colonization by suspected pathogens. Information of this type will provide valuable knowledge to our understanding of periodontal diseases and possibly provide a basis for a rational approach to immunologic prevention of periodontal diseases.

该中心的主要目标是制定模式， 牙周病的诊断、治疗和预防。 一 一系列相关的临床、微生物学和免疫学 为实现这些目标进行了研究。 的 临床部分旨在检测早期疾病活动 利用高灵敏度的检测方法， 已被证明具有高灵敏度的恒力探针 和可复制性。 微生物和免疫学研究是 旨在研究难治性和 化脓性牙周病和定义 特定微生物与宿主之间的相互关系 免疫反应 将进行临床测量，以验证 各个站点都处于积极的进展状态， 在治疗前的疾病和治疗后的缓解状态。 微生物群的组成和免疫反应将是 对每个患者进行纵向检查，以确定总体 不同治疗方案的效果。 测定 不同牙周治疗方法对临床疗效的影响 宿主的微生物群和免疫反应，应该会导致更多的 合理的治疗方法。 通过 深入调查相互关联因素的多样性 与牙周病过程相关，它希望， 为合理防治奠定基础。 松鼠猴研究的目的是 为了开发一种体内模型， 牙周病原体（例如，B。gingivalis、齿突刺蛾H. 放线菌属），然后检查的影响， 殖民地的免疫。 具体目标是：（1） 在一种或多种龈下细菌之间建立关联 (e.g., B。（2）确定与疾病的关系; 对可疑牙周病原体的免疫力;（3）调查 感染和口腔定植的猴子的机制 病原体;（4）识别和表征来自 通常被感染和免疫的病原菌 免疫猴制备抗体;（5）检测免疫对 病原菌在龈下菌斑的定植。 从这些研究中，涉及疾病的微生物群以及 将确定对这些细菌的体液免疫应答。 一 龈下菌斑定植模型 将被开发。 该模型将用于研究 体液免疫应答调节口腔黏膜免疫的潜力 疑似病原体的定植。 此类信息将 为我们了解牙周病提供了有价值的知识 疾病，并可能提供一个合理的方法， 牙周病的免疫预防。