ROLE OF ENDOGENOUS AND RECOMBINANT RETROVIRUSES IN LEUKEMIA AND DIFFERENTIATION

内源性和重组逆转录病毒在白血病和分化中的作用

• 批准号: 3818158
• 负责人: B W CHESEBRO
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3818158
• 关键词: Friend leukemia; Friend virus; cellular oncology; gene expression; genetic recombination; genetic strain; hemolytic anemia; laboratory mouse; molecular cloning; molecular oncology; newborn animals; point mutation; protein engineering; protein sequence; splenomegaly; viral leukemia; viral leukemogenesis; virus envelope; virus genetics; virus infection mechanism

The goal of this work is to study mechanisms of pathogenesis of murine retroviruses. Previous studies showed that two closely related variants of Friend murine leukemia viruses differed in their ability to induce hemolytic anemia and splenomegaly shortly after inoculation into newborn mice. In vitro construction of recombinants between these strains demonstrated that this difference was due to differences in the viral envelope gene. DNA sequencing has revealed only 2 sequence differences between these strains, and site-specific mutagenesis has now identified a single amino acid responsible for this alteration in pathogenesis.

本工作的目的是研究其发病机制