ROLE OF ENDOGENOUS AND RECOMBINANT RETROVIRUSES IN LEUKEMIA AND DIFFERENTIATION

本工作的目的是研究黄褐斑的发病机制。 小鼠逆转录病毒。之前的研究表明，有两个密切相关的 Friend鼠白血病病毒的相关变异株在 短期内引起溶血性贫血和脾肿大的能力 接种到新生小鼠体内后。体外构建小鼠胸腺细胞系 这些菌株之间的重组证明了这一点 差异是由于病毒包膜基因的差异所致。脱氧核糖核酸 测序显示，它们之间只有2个序列差异 菌株，定点突变现在已经识别出单一的 氨基酸在病程中引起这种变化的氨基酸。

The goal of this work is to study mechanisms of pathogenesis of murine retroviruses. Previous studies showed that two closely related variants of Friend murine leukemia viruses differed in their ability to induce hemolytic anemia and splenomegaly shortly after inoculation into newborn mice. In vitro construction of recombinants between these strains demonstrated that this difference was due to differences in the viral envelope gene. DNA sequencing has revealed only 2 sequence differences between these strains, and site-specific mutagenesis has now identified a single amino acid responsible for this alteration in pathogenesis.