ROLE OF ENDOGENOUS AND RECOMBINANT RETROVIRUSES IN LEUKEMIA AND DIFFERENTIATION

内源性和重组逆转录病毒在白血病和分化中的作用

• 批准号: 4688425
• 负责人: B W CHESEBRO
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4688425
• 关键词: Friend leukemia; Friend virus; cell differentiation; erythroleukemia; gene expression; genetic recombination; lymphocytic leukemia; molecular cloning; monoclonal antibody; myelogenous leukemia; newborn animals; viral leukemia; viral leukemogenesis; virus RNA; virus classification; virus envelope; virus genetics; virus infection mechanism; virus virus interaction

A new focal immunofluorescence assay (FIA) was developed for use in assaying and biologically cloning retroviruses infecting live cell monolayers. By using monoclonal antibodies with specificity for only certain strains of murine retrovirus, it was possible to measure interference to superinfection of chronically infected cells. Surprisingly the patterns of interference varied markedly when the same viruses were compared in different cell lines. These results suggested that specificity of cellular receptors for retroviruses could vary widely in different cell lines. Furthermore, the patterns of interference seen in one particular cell line could not be explained by the presence of a small number of different receptor types. The FIA has also been used to compare virus replication and recombinant MCF virus generation in mice inoculated with a low virulence variant Friend murine leukemia virus strain (F-MuLV-B3). Results indicated that B3 was not defective in replication or recombinant MCF virus generation, nevertheless in vivo leukemic transformation as detected by splenomegaly or lymphadenopathy was delayed by several months compared to the parental virus strain (F-MuLV 57). Preliminary results suggest that strains B3 and 57 may not differ in ability to transform hemopoietic cells, but rather in their ability to induce hemolytic anemia with compensatory splenomegaly early after inoculation.

本研究建立了一种新的局部免疫荧光试验（FIA）， 检测和生物学克隆感染活细胞的逆转录病毒 单层。 通过使用仅对以下物质具有特异性的单克隆抗体， 某些鼠逆转录病毒株，有可能测量 干扰慢性感染细胞的重复感染。 人惊讶 当相同的病毒被感染时， 在不同的细胞系中进行比较。 这些结果表明，特异性 逆转录病毒的细胞受体在不同的细胞中可能有很大的不同 线 此外，在一个特定的干扰模式中， 细胞系不能用少量细胞的存在来解释 不同的受体类型 FIA也被用于比较病毒复制和重组MCF 用低毒力变体Friend接种的小鼠中的病毒生成 小鼠白血病病毒株（F-MuLV-B3）。 结果表明，B3是 在复制或重组MCF病毒产生中没有缺陷， 然而，如通过脾肿大检测到的体内白血病转化或 淋巴结病比父母推迟了几个月 病毒株（F-MuLV 57）。 初步结果表明，菌株B3和 57在转化造血细胞的能力上可能没有差异，但在 它们诱导溶血性贫血伴代偿性脾肿大的能力 接种后早期。