ROLE OF ENDOGENOUS AND RECOMBINANT RETROVIRUSES IN LEUKEMIA AND DIFFERENTIATION

建立了一种新的斑点免疫荧光分析方法(FIA)，用于 感染活细胞的逆转录病毒的检测和生物克隆 单层。通过使用仅具有特异性的单抗 某些鼠类逆转录病毒株，有可能测量到 对慢性感染细胞重复感染的干预。令人惊讶的是 当相同的病毒被感染时，干扰模式明显不同 在不同的细胞系中进行比较。这些结果表明，特异性 逆转录病毒的细胞受体在不同的细胞中可能有很大的不同 台词。此外，在一种特殊情况下看到的干扰模式 细胞系不能用少量的存在解释 不同的受体类型。 FIA还被用于比较病毒复制和重组MCF 小鼠接种低毒变异体Friend后的病毒生成 小鼠白血病病毒F-MuLV-B3株。结果表明，B3是 在复制或重组MCF病毒生成方面没有缺陷， 然而，体内白血病的转化是通过脾肿大或 与父母相比，淋巴结病延迟了几个月 病毒株(F-MuLV 57)。初步结果表明，菌株B3和 57在转化造血细胞的能力上可能没有区别，但在 代偿性脾肿大诱发溶血性贫血的能力 接种后早期接种。

A new focal immunofluorescence assay (FIA) was developed for use in assaying and biologically cloning retroviruses infecting live cell monolayers. By using monoclonal antibodies with specificity for only certain strains of murine retrovirus, it was possible to measure interference to superinfection of chronically infected cells. Surprisingly the patterns of interference varied markedly when the same viruses were compared in different cell lines. These results suggested that specificity of cellular receptors for retroviruses could vary widely in different cell lines. Furthermore, the patterns of interference seen in one particular cell line could not be explained by the presence of a small number of different receptor types. The FIA has also been used to compare virus replication and recombinant MCF virus generation in mice inoculated with a low virulence variant Friend murine leukemia virus strain (F-MuLV-B3). Results indicated that B3 was not defective in replication or recombinant MCF virus generation, nevertheless in vivo leukemic transformation as detected by splenomegaly or lymphadenopathy was delayed by several months compared to the parental virus strain (F-MuLV 57). Preliminary results suggest that strains B3 and 57 may not differ in ability to transform hemopoietic cells, but rather in their ability to induce hemolytic anemia with compensatory splenomegaly early after inoculation.