REGULATION OF INTERLEUKIN 2 GENE EXPRESSION IN LYMPHOID AND NONLYMPHOID CELLS

淋巴细胞和非淋巴细胞中白细胞介素 2 基因表达的调节

• 批准号: 3821518
• 负责人: N J HOLBROOK
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3821518
• 关键词: Retroviridae; T lymphocyte; aging; cell growth regulation; cell type; cytogenetics; epithelium; fibroblasts; gene expression; genetic manipulation; genetic mapping; immune response genes; interleukin 2; messenger RNA

This project is focused at understanding the basic control mechanisms responsible for expression of the human interleukin 2 (IL-2) gene. Particular items addressed are as follows: 1) Expression os the human IL-2 gene in various cell types - Ordinarily, human IL-2 is expressed only in T cells where it is under tight inducible control. We have stably transfected the human IL-2 gene into mouse fibroblasts and a human epithelial cell line. In both cases the gene is constitutively expressed. The expressed mRNA is not of the expected size, however. RNA mapping experiments have been used to define the basis for its altered expression. 2) The mechanism for activation of IL-2 expression in MLA 144 cells by a retroviral insertion - The role of the viral regulatory elements in influencing IL-2 expression in transient systems have been investigated. 3) Role of 3' sequences in controlling Il-2 expression have been examined.

这个项目的重点是了解基本的控制 人白细胞介素2的表达机制 （IL-2）基因。 具体涉及的项目如下：1） 人IL-2基因在不同细胞类型中的表达- 通常，人IL-2仅在T细胞中表达，在T细胞中它是 在严格的诱导控制下 我们已经稳定地转染了 人IL-2基因导入小鼠成纤维细胞和人上皮细胞 细胞系 在这两种情况下，基因都是组成型表达的。 的 然而，表达的mRNA不是预期的大小。 RNA 映射实验已被用来定义其基础， 改变表达。 2)IL-2的激活机制 通过逆转录病毒插入在MLA 144细胞中的表达- 病毒调节元件在影响IL-2表达中的作用 已经研究了瞬态系统。 3)3'序列的作用 在控制IL-2表达中的作用。