REGULATION OF INTERLEUKIN 2 GENE EXPRESSION IN LYMPHOID AND NONLYMPHOID CELLS

本项目的重点是了解基本控制 人白细胞介素2的表达机制 (IL-2)基因。具体事项如下：1) 人IL-2基因在不同细胞类型中的表达 正常情况下，人IL-2只在T细胞中表达 在严格的可诱导控制下。我们已经稳定地转导了 人IL-2基因导入小鼠成纤维细胞和人上皮细胞 细胞系。在这两种情况下，基因都是结构性表达的。这个 然而，表达的mRNA并不是预期的大小。核糖核酸 测绘实验已经被用来确定ITS的基础 表情改变了。2)IL-2的激活机制 逆转录病毒插入在MLA 144细胞中的表达--作用 影响IL-2表达的病毒调控元件 对暂态系统进行了研究。3)3‘端序列的作用 在控制IL-2的表达方面已经进行了研究。

This project is focused at understanding the basic control mechanisms responsible for expression of the human interleukin 2 (IL-2) gene. Particular items addressed are as follows: 1) Expression os the human IL-2 gene in various cell types - Ordinarily, human IL-2 is expressed only in T cells where it is under tight inducible control. We have stably transfected the human IL-2 gene into mouse fibroblasts and a human epithelial cell line. In both cases the gene is constitutively expressed. The expressed mRNA is not of the expected size, however. RNA mapping experiments have been used to define the basis for its altered expression. 2) The mechanism for activation of IL-2 expression in MLA 144 cells by a retroviral insertion - The role of the viral regulatory elements in influencing IL-2 expression in transient systems have been investigated. 3) Role of 3' sequences in controlling Il-2 expression have been examined.