TRANSCRIPTIONAL CONTROL ELEMENTS IN THE GIBBON APE LEUKEMIA VIRUS LTR

长臂猿白血病病毒 LTR 中的转录控制元件

• 批准号: 3821517
• 负责人: N J HOLBROOK
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3821517
• 关键词: Rous sarcoma virus; aging; cell growth regulation; croton oil; deoxyribonuclease I; gene expression; genetic regulation; genetic transcription; human T cell lymphotropic virus type 1; leukemia virus; molecular genetics; neoplasm /cancer genetics; nucleic acid sequence; simian virus 40; tumor promoters

Studies were undertaken to localize precisely the transcriptional enhancer elements within the long terminal repeats (LTRs) of different gibbon ape leukemia virus (GALV) strains and identify the cellular factor which binds to the enhancer region. 1) We have shown that the major element responsible for enhancer activity of the GALV LTR is contained within a 12 bp sequence of a 45 bp repeated DNA segment. 2) Using exonuclease II and DNAse I footprinting techniques we have identified a nuclear factor which binds specifically to the 123 bp enhancer sequence. It is present in cells which express GALV but not in cells which express GALV poorly. 3) We have demonstrated that the tumor promoter phorbol myristate acetate can enhance transcriptional activity of the GALV LTR 50-100 fold in various lymphoid cell lines. It also enhances expression of several other viral transcriptional elements including SV40, human T cell lymphotrophic virus type I, and Rous sarcoma virus, suggesting it works via a generalized mechanism. For practical purposes, PMA provides a convenient means for increasing transient expression of foreign DNA in lymphoid cells.

进行了研究以精确定位转录本 长末端内的增强子元件重复(LTrs) 长臂猿白血病病毒(Galv)不同毒株的鉴定 结合到增强子区域的细胞因子。 1)我们已经证明，负责 Galv LTR的增强子活性被控制在12个碱基对以内 45个碱基重复的DNA片段的序列。 2)使用核酸外切酶II和DNase I足迹技术 已经确定了一种核因子，它特定地与 123个碱基的增强子序列。它存在于细胞中，表达 Galv，但在表达Galv较差的细胞中不表达。 3)我们已经证明了肿瘤促进剂佛波醇 肉豆蔻酸盐可以增强细胞的转录活性。 在不同的淋巴样细胞系中，Galv LTR50-100倍。它还 增强其他几种病毒转录产物的表达 包括SV40，人T细胞趋化病毒I型， 和劳斯肉瘤病毒，表明它是通过一种泛发性的 机制。出于实用目的，PMA提供了一种方便的 提高外源DNA瞬时表达的方法 淋巴样细胞。