SATURATION MAPPING OF HUMAN CHROMOSOME 3

这个项目的目标是产生详细的物理和 人类2号和3号染色体多态性标记的遗传图谱。 的 目前2号和3号染色体的图谱仅具有有限数量的标记 在物理和遗传图谱上都有几个大的缺口。 该计划项目生成的物理地图将基于 辐射减少杂种和遗传图谱分析CEPH的结果 家族DNA 产生的遗传标记将全部基于PCR， 由高度多态性的标记组成，例如用简单的二，三， 和四核苷酸重复序列，以及L1序列的多聚腺苷酸序列 作为这些染色体的特异性转录序列。 此外 程序将设计一种方法，用Line对每条染色体进行全局映射 元素，并将该图与遗传和辐射混合图相关联。 为了实现这些目标，该计划包括4个项目和一个核心 联系研究以及行政核心。 这些高度 相互依存的项目将导致放置600个非常有用的 2号和3号染色体上的标记。 所述标记 因为它们是高度多态的，所以被称为高度有用的，基于PCR 并提供给所有研究人员，并映射在物理和 基因图谱 生成的物理图将在2 cM处完成 水平，在许多地区1厘米的距离将实现。 物理 每个染色体的图谱将具有大约1个标记/兆碱基。 这 标记物和细胞试剂的框架面板将用于指导 分离2号和3号染色体的有序克隆， 对这些染色体的闭合有用。 多态性标记将是 直接适用于鉴定疾病位点附近的标记。

The goal of this program project is to produce detailed physical and genetic maps of polymorphic markers for human chromosomes 2 and 3. The current maps of chromosomes 2 and 3 only have a limited number of markers and there are several large gaps on both the physical and genetic maps. The physical map generated by the program project will be based on radiation reduction hybrids and the genetic map a result of analyzing CEPH family DNA. The genetic markers generated will all be PCR based and consist of highly polymorphic markers such as found with simple di-, tri-, and tetranucleotide repeats and in the poly A tract of L1 sequences as well as transcribed sequences specific for these chromosomes. In addition the program will devise a method for globally mapping each chromosome with Line elements and correlating this map to the genetic and radiation hybrid map. To accomplish these goals, the program consists of 4 projects and a core for linkage studies as well as an administrative core. These highly interdependent projects will result in the placement of 600 highly useful markers on chromosomes 2 and 3 over the grant period. The markers are termed highly useful since they will be highly polymorphic, be based on PCR and available to all investigators, and be mapped on both physical and genetic maps. The physical map generated will be complete at the 2 cM level and in many regions 1 cM distances will be achieved. The physical map of each chromosome will have approximately 1 marker per megabase. This framework panel of markers and cell reagents will be used to direct the isolation of ordered clones for chromosomes 2 and 3 and will be especially useful for closure on these chromosomes. The polymorphic markers will be immediately applicable for identifying markers adjacent to disease loci.