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SUBCELLULAR COMPOSITION OF THE PANCREATIC ISLETS OF LANGERHANS

SUBCELLULAR COMPOSITION OF THE PANCREATIC ISLETS OF LANGERHANS
朗厄汉斯胰岛的亚细胞组成
批准号：
3852995
负责人：
R LEAPMAN
金额：
$0.0万
依托单位：
NATIONAL CENTER FOR RESEARCH RESOURCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3852995
关键词：
animal tissue biomedical equipment development cryoscience method development pancreatic islets scanning electron microscopy transmission electron microscopy

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中文摘要
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英文摘要
Rapid-freezing and cryosectioning techniques have been established for obtaining specimens from isolated, incubated rat pancreatic islets of Langerhans. The existing freezing machine was modified to function at liquid nitrogen temperatures, so that it was possible to freeze individual islets mounted with physiological buffer on a gold freezing 'hat.' The cryoultramicrotome was set up with a diamond trimming tool, a 35 degree diamond knife, and a 12-kV antistatic gun to prevent the tissue from charging during cryosectioning. With these modifications, it is now possible to obtain high-quality cryosections on which useful experiments can be performed, both in the Hitachi TEM-STEM and in the VG Microscopes field-emission STEM.
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