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SUBCELLULAR COMPOSITION OF THE PANCREATIC ISLETS OF LANGERHANS

SUBCELLULAR COMPOSITION OF THE PANCREATIC ISLETS OF LANGERHANS
朗厄汉斯胰岛的亚细胞组成
批准号：
3852995
负责人：
R LEAPMAN
金额：
$0.0万
依托单位：
NATIONAL CENTER FOR RESEARCH RESOURCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3852995
关键词：
animal tissue biomedical equipment development cryoscience method development pancreatic islets scanning electron microscopy transmission electron microscopy

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项目成果

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相关文献

中文摘要
翻译
已经建立了快速冷冻和冷冻切割技术 从分离培养的大鼠胰岛中获取标本 朗格汉斯。对现有的冷冻机进行了改造，使之能在 液氮温度，这样就有可能冻结 安装了生理缓冲器的单个胰岛冻结黄金 “帽子。”冰冻切割机是用一种钻石修边工具-- 35度钻石刀，12千伏防静电枪，防止纸巾 在冷冻过程中不能充电。经过这些修改，现在是 有可能获得高质量的冷冻切片，在其上进行有用的实验 可以在日立电子显微镜和VG显微镜上进行 场发射STEM。
英文摘要
Rapid-freezing and cryosectioning techniques have been established for obtaining specimens from isolated, incubated rat pancreatic islets of Langerhans. The existing freezing machine was modified to function at liquid nitrogen temperatures, so that it was possible to freeze individual islets mounted with physiological buffer on a gold freezing 'hat.' The cryoultramicrotome was set up with a diamond trimming tool, a 35 degree diamond knife, and a 12-kV antistatic gun to prevent the tissue from charging during cryosectioning. With these modifications, it is now possible to obtain high-quality cryosections on which useful experiments can be performed, both in the Hitachi TEM-STEM and in the VG Microscopes field-emission STEM.
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