MAPPING PROTEIN DISTRIBUTIONS IN EPIDERMIS BY ELECTRON ENERGY LOSS SPECTROSCOPY

为了了解鳞状上皮细胞在 终末分化，重要的是要确定分布的 关键蛋白质在连续的阶段。 以补充 免疫细胞化学，我们已经进行了基于EM的元素映射， 新生小鼠表皮和前胃中硫和氮。 能量过滤透射电子显微镜（EFTEM）图像显示， 在圆形细胞质颗粒中和在 皮层细胞被膜（CE），与兜甲蛋白（约6%）相关 半胱氨酸）。电子能量损失谱分析证实 这些观察结果，支持了推断的半导体产品 L-颗粒与CE的关系。 附近的其他矿床 表皮CE具有类似的硫含量，但不标记 抗兜甲蛋白抗体，从而表明存在另一种富含S 蛋白

To understand the changes undergone by squamous epithelial cells during terminal differentiation, it is important to determine distributions of key proteins at successive stages. As a complement to immuno-cytochemistry, we have performed EM-based elemental mapping of sulfur and nitrogen in newborn mouse epidermis and forestomach. Energy-filtered transmission electron microscopy (EFTEM) images reveal high sulfur concentrations in round cytoplasmic granules and in the cornified cell envelope (CE), correlating with loricrin (about 6% cysteine). Electron energy-loss spectroscopy (EELS) analysis confirms these observations, supporting the inferred precursor-product relationship between L-granules and the CE. Other deposits near the epidermal CE have a similar sulfur content, but do not label with antiloricrin antibodies, thus suggesting the presence of another S-rich protein.