PHYSICS OF IONIC CHANNELS AND OTHER PROTEINS WITH AQUEOUS CAVITIES

通过渗透胁迫的使用，已经发现大约有60 当血红蛋白从“紧张”状态转换时，水分子会与其结合。 脱氧为“松弛”的氧合形式。看起来这水应该是 与氧或其他变构一样被视为配基物种 效应器。 金黄色葡萄球菌的α毒素跨膜通道 被重组成双层膜并在渗透压力下 提供600个水分子进出此区域的信息 通道在外加电压下打开和关闭时。这个频道 似乎也有不同的PH值敏感性从两侧的 薄膜。 褐鼠肥大细胞分泌颗粒释放后的肿胀 似乎受到水合/脱水事件的控制，这些事件可以 由热力学分析描述，类似于发展到 体外大分子组装分析。肿胀的偶合 伴随着分泌物与反离子的交换 多糖基质表明在体内和在 在体外过程中。

Through the use of osmotic stress, it has been found that some 60 water molecules bind to hemoglobin when it switches from the "tense" deoxy to the "relaxed" oxy forms. It appears that this water should be treated as a liganding species much as oxygen or other allosteric effectors. The alpha-toxin trans-membrane channel from Staphylococcus aureus has been reconstituted into bilayer membranes and osmotically stressed to provide the information that 600 water molecules enter and leave this channel when it opens and closes under applied voltage. This channel appears also to have different pH sensitivity from each side of the membrane. Swelling of secretory granules after release from beige mouse mast cells appears to be governed by hydration/dehydration events that can be described by thermodynamic analysis similar to that developed to analyze macromolecular assembly in vitro. The coupling of swelling concomitant to secretion with the exchange of counterions to the polysaccharide matrix suggests a further parallel between in vivo and in vitro processes.