RENAL LESIONS IN THE ABLATION MODEL--ROLE OF GROWTH FACTORS

消融模型中的肾损伤——生长因子的作用

• 批准号: 3855328
• 负责人: L J STRIKER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3855328
• 关键词: autoradiography; cell growth regulation; chronic renal failure; growth factor; kidney cell; laboratory rat; nephrectomy; nephrosclerosis; radiotracer; renal glomerulus; tritium

Subtotal nephrectomy in rats, the so-called ablation model, leads to progressive destruction of the kidney and the development of chronic renal failure. We postulated that the progressive glomerular lesions were due to an early increase in the turnover of glomerular resident cells. This abnormal glomerular growth results in an increase in the glomerular volume which is detectable using morphometric measurements. We have performed autoradiographic studies, using (3)H-thymidine, and found that within two days following subtotal nephrectomy there was an increase in the glomerular mitotic index, as well as an increase in the turnover of the cells forming the arterial wall. These findings suggest that a dysregulation of cell growth is an early event in the development of glomerulosclerosis following reduction in renal mass.

大鼠肾次全切除术，即所谓的消融模型，导致 肾脏的进行性破坏和慢性肾功能衰竭的发展 失败 我们推测进行性肾小球病变是由于 肾小球驻留细胞更新的早期增加。 这 异常肾小球生长导致肾小球体积增加 其可使用形态测量法检测。 我们已执行 放射自显影研究，使用（3）H-胸苷，并发现，在两个 在肾大部切除术后30天， 有丝分裂指数，以及细胞形成的周转增加， 动脉壁 这些发现表明， 生长是肾小球硬化症发展的早期事件， 肾脏质量减少。