REAL-TIME HIGH-PERFORMANCE CONFOCAL LASER SCANNING OPTICAL MICROSCOPE

实时高性能共焦激光扫描光学显微镜

• 批准号: 3874173
• 负责人: S GOLDSTEIN
• 金额: --
• 依托单位: NATIONAL CENTER FOR RESEARCH RESOURCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3874173
• 关键词: biomedical equipment development; confocal scanning microscopy; fluorescence microscopy; image enhancement; lasers

A no-moving-parts, 30-frames-per-second, laser beam scanning confocal reflected light microscope has been developed. In principle the technique can be extended to fluorescence and transmission light microscopy. Acousto-optic beam deflectors, controlled by all-digital electronics, move a laser beam in a 512line interlaced raster. The light enters an inverted microscope through the epi-fluorescence side camera port, and is imaged at the object by the microscope objective. Reflected light returns through the objective, out the camera port, and is imaged onto the photocathode of an Image Dissector Tube (IDT) . Confocality is provided by raster-scanning the IDT aperture coincident with the congruent image of the laser beam incident on the object. Realtime, jitter-free reflected light images of a variety of biological objects have been produced. Computer-controlled alignment of the laser scan and IDT is performed in several seconds.

一种非移动部件、每秒30帧的激光扫描共焦装置 反射式光学显微镜已经研制成功。原则上讲，这项技术 可扩展到荧光显微镜和透射光显微镜。 声光光束偏转器，由全数字电子控制，移动 512行隔行扫描栅格中的激光束。光线进入一个倒置的 通过外荧光侧面的摄像头端口进行成像，并在 通过显微镜物镜观察物体。反射的光通过 物镜从相机端口出来，并成像到光电阴极上 一种像分光管(IDT)。共焦度由栅格扫描提供 与激光光束的相干像符合的IDT孔径 发生在物体上的事件。实时、无抖动的反射光图像 各种各样的生物物体已经被生产出来。计算机控制 激光扫描和IDT的对准在几秒钟内完成。