REAL-TIME HIGH-PERFORMANCE CONFOCAL LASER SCANNING OPTICAL MICROSCOPE

一种每秒30帧的非移动部件激光扫描共焦装置 反射式光学显微镜已经研制成功。原则上讲，这项技术 可扩展到荧光显微镜和透射光显微镜。 声光光束偏转器，由所有数字电子设备控制，移动 一束512行的激光隔行扫描8.5 mm正方形栅格。光线照进来了 倒置显微镜通过侧面摄像头端口，并在 用显微镜物镜观察物体。反射的光通过 物镜，通过分束器从相机端口出来，并成像到 像分光管(IDT)的光电阴极。提供共焦功能 通过光栅式扫描，使IDT孔径与同源图像重合 激光入射到物体上。实时无抖动反射 各种生物物体的光图像已经被制作出来。 激光扫描和IDT的计算机控制对准在 几秒钟。

A no-moving-parts, 30 frames per second, laser beam scanning confocal reflected light microscope has been developed. In principle the technique can be extended to fluorescence and transmission light microscopy. Acousto-optic beam deflectors, controlled by all digital electronics, move a laser beam in a 512 line interlaced 8.5mm square raster. The light enters an inverted microscope through the side camera port, and is imaged at the object by the microscope objective. Reflected light returns through the objective, out the camera port, through a beam splitter, and is imaged onto the photocathode of an Image Dissector Tube (IDT) . Confocality is provided by raster scanning the IDT aperture coincident with the congruent image of the laser beam incident on the object. Real time jitter-free reflected light images-of a variety of biological objects have been produced. Computer controlled alignment of the laser scan and IDT is performed in several seconds.